The Flex caddy allows the Thermocycler GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Thermocycler. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Thermocycler Module. The module is available with or without the Flex Caddy and Calibration tool: Thermocycler Module GEN2
Detail
The Flex caddy allows the Thermocycler GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Thermocycler. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Thermocycler Module. The module is available with or without the Flex Caddy and Calibration tool: Thermocycler Module GEN2
p53, also known as tumor protein 53 or TP53, is a tumor suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumors, including those of the breast, ovarian, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
Propargyl-PEG3-CH2CO2H is an PEG linker with an alkyne and carboxylic acid set of functional groups. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed.
Document
Propargyl-PEG3-CH2CO2H is an PEG linker with an alkyne and carboxylic acid set of functional groups. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Plasmid Maxi preparation
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/B, 8 layers
Membrane aperture
1.0 μm
Maximum binding yield of plasmid
1 mg
Maximum yield of alcohol mediated binding
5 mg
Plasmid yield
Up to 1mg
Single liquid carrying capacity of column
20 ml
Minimum elution volume
800 μl
Withstand centrifugal force
3,000-5,000 x g
Centrifuge
Low speed centrifuge for 50ml centrifuge tubes, >3000 x g, swing-out Rotor
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13123
HiPure DNA Maxi Column III (8 x GF/B)with 50ml Collection Tubes
100/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
