The Flex caddy allows the Thermocycler GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Thermocycler. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Thermocycler Module. The module is available with or without the Flex Caddy and Calibration tool: Thermocycler Module GEN2
Detail
The Flex caddy allows the Thermocycler GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Thermocycler. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Thermocycler Module. The module is available with or without the Flex Caddy and Calibration tool: Thermocycler Module GEN2
Other Products
Total Dietary Fiber Assay Kit
Product Info
Document
Product Info
K-TDFR-200A
SKU: 700004347
Content:
100 assays / 200 assays
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Dietary Fiber
Assay Format:
Enzymatic
Detection Method:
Gravimetric
Signal Response:
Increase
Limit of Detection:
0.5 g/100 g
Total Assay Time:
~ 100 min
Application examples:
Food ingredients, food products and other materials.
Method recognition:
AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19, CODEX Method Type I and GB Standard 5009.88-2014
The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiberaccording to AOAC and AACC approved methods.
Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.
Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399. 2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370. 3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Two separate methods are described in the associated assay protocol:
METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER Based on AACC method 32-05.01 and AOAC Method 985.29.
Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.
DMSO resistant peelable heat sealing foil which is suitable for low temperature storage, high temperature uses and PCR testing.
Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective
Our PeelASeal Foil Super is a laminate seal compatible with all plate types
It can be removed by peeling, even with a plate which has been removed directly from -80°C storage
PeelASeal Foil Super forms a complete seal to a plate enabling very low temperature uses, including very low temperature storage, and high temperature uses, such as PCR
The seal demonstrates high solvent resistance (including DMSO) and can be utilized for short term compound storage at room temperature
The seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
Usages： For determination of coliform confirmatory test for multi-tube fermentation.
Principle: Peptone provide carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
Formulation (per liter): Peptone: 10g Lactose: 10g Ox bile powder: 20g Brilliant green: 0.0133g Final pH7.2 ± 0.2
How to use: 1.Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121 ℃ for 15min, cool to room temperature and set aside. 2.Sample handling and dilution. Slightly. 3.Transplanted LST broth tube to brilliant green lactose bile broth tube by using a inoculating loop. 4.the tube was placed in an incubator culture at 36 ± 1 ℃ for 24h. 5.Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
Quality control: Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows: Bacterial Name Bacterial No. Growth Status Gassing Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
