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Format Lyophilized In Vitro Diagnostic Test Kits With High Sensitivity

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Detail

Product Description

Format Lyophilized In Vitro Diagnostic With High Sensitivity

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

ParametersDetails
Product NameRNA Isothermal Amplification Kit Basic
ManufacturerAmp-future
Storage Temperature-20°C
Kit ComponentsEnzymes, Buffers ,Reagents
Packaging48 Tests/box
Detection Limit500-1000copies/µL
ShippingICE
Test Time5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(BASIC type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Other Products

Basic Nucleic Acid DNA Amplification Kit 48 Reactions 20mins

Product Description

Unlocking The Potential Of Nucleic Acid Amplification With DNA Amplification Kit (Basic)

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

ParametersDetails
Product NameDNA Isothermal Amplification Kit Basic
ManufacturerAmp-future
Storage Temperature-20°C
Kit ComponentsEnzymes, Buffers ,Reagents
Packaging48 Tests/box
Detection Limit500-1000copies/µL
ShippingICE
Test Time5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

TwistAmp® exo

Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.

Total RNA Purification Kits

Overview

  • Extract high quality & quantity total RNA including miRNA
  • No phenol step required; isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
  • Available in a variety of formats to suit your needs
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #NameElution VolumePlasma/SerumUrineCell-Culture Media
55000Plasma/Serum RNA Purification Mini Kit10 – 25 µL50 µL – 1 mL250 µL – 1 mL5 – 10 mL
35300Total RNA Purification Micro Kit20 – 50 µL1 – 4 mL2 – 10 mL10 – 20 mL
17200Total RNA Purification Kit50 – 100 µL4 – 10 mL11 – 30 mL20 – 35 mL

Details

Supporting Data

Figure 1 / 13

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Figure 1. High Quality of Isolated RNA with Complete Size Range.
Figure 1. High Quality of Isolated RNA with Complete Size Range.
Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction.
Figure 3. High Quality of RNA from a Diverse Range of Inputs.
Figure 4. Great Isolation Sensitivity.
Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA.
Figure 6. Effective and Consistent Detection of miRNA from Plasma.
Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency.
Figure 8. Better Diversity of miRNA Detected from Plasma.
Figure 9. Better Diversity of miRNA Detected from HeLa Cells.
Figure 10. Total RNA Purification 96-Well Kit
Figure 11. Total RNA Purification 96-Well Kit
Figure 12. Total RNA Purification 96-Well Kit
Figure 13. Total RNA Purification 96-Well Kit

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Click for expanded view

Kit Specifications
Maximum Binding Capacity Per Well50 μg
Maximum Loading Volume Per Well500 μL
Size of RNA PurifiedAll sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:
Animal Cells1 x 106 cells
Animal Tissues10 mg
Blood100 μL
Plasma/Serum150 μL
Bacteria1 x 109 cells
Yeast1 x 108 cells
Fungi40 mg
Plant Tissues40 mg
Time to Complete 96 Purifications30 minutes
Average Yields*
HeLa Cells (1 x 106 cells)15 μg
E. coli (1 x 109 cells)50 μg

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

ComponentCat. 17200 (50 preps)Cat. 37500 (100 preps)Cat. 17250 (250 preps)Cat. 17270 (500 preps)Cat. 24300 (192 preps)Cat. 24370 (576 preps)Cat. 24350 (192 preps)Cat. 24380 (576 preps)
Buffer RL40 mL2 x 40 mL175 mL350 mL2 x 40 mL350 mL2 x 40 mL350 mL
Wash Solution A38 mL2 x 38 mL148 mL1 x 148 mL
1 x 74 mL
2 x 38 mL1 x 74 mL
1 x 148 mL
2 x 38 mL1 x 74 mL
1 x 148 mL
Elution Solution A6 mL2 x 6 mL30 mL60 mL2 x 20 mL60 mL2 x 20 mL60 mL
Mini Spin Columns50100250500
96-Well Isolation Plate26
96-Well Isolation Plate (Deep Well)26
Adhesive Tape412412
Collection Tubes50100250500
96-Well Collection Plate26
96-Well Collection Plate (Deep Well)26
Elution Tubes (1.7 mL)50100250500
96-Well Elution Plate26
96-Well Elution Plate (Deep Well)26
Product Insert11111111