Overview
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Sixteen discrete fragments ranging from 100 bp to 5000 bp
- Higher intensity reference bands at 500 bp and 1000 bp
The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100 bp to 5000 bp with higher intensity reference bands at 500 bp and 1000 bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
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FullRanger 100 bp DNA Ladder (Cat# 11800) – 100 loads
Ladder Properties:
• Sixteen discrete bands, ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp
| Fragment | Size (bp) | Mass (ng) |
| 1 | 5000 | 55 |
| 2 | 4000 | 44 |
| 3 | 3000 | 33 |
| 4 | 2500 | 28 |
| 5 | 2000 | 22 |
| 6 | 1500 | 17 |
| 7 | 1000 | 50 |
| 8 | 900 | 35 |
| 9 | 800 | 31 |
| 10 | 700 | 43 |
| 11 | 600 | 24 |
| 12 | 500 | 56 |
| 13 | 400 | 16 |
| 14 | 300 | 22 |
| 15 | 200 | 15 |
| 16 | 100 | 11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
