Features: 

1.Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.

2.Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.

3.Automatic lid lock, LCD display, It can store 100 programs. super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

4.Adapters are available by experiment requirements.

5.3 tiers protection steel cover, safe and reliable. 

6.IEC CE EMC certificate.

 TD5 Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×250ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	570×400×320mm
Net /Gross weight(Kg)	42KG /47KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TD5 

Order No	Rotor	Max	Volume(ml)	Max RCF(×g)
		Speed(r/min)
D5-1	Swing rotor(round	4000	4×250ml	2880
	cup)
D5-2	Swing buckets	4000	4×16×7ml	2860
D5-3		4000	4×16×5ml	2470
D5-4		4000	4×13×10ml	2830
D5-5		5000	4×1×50ml	4730
	Swing rotor
D5-6			4×1×100ml	4730
D5-7			4×2×100ml	3020
D5-8			4×2×50ml	3020
D5-9			4×4×15ml	3020
D5-10			4×6×15ml	3020
D5-11			4×4×10/7	2770
D5-12			4×4×5 vacuum tube	2360
		#######	adapters
D5-13			4×6×10/7	2770
D5-14			4×6×5 vacuum tube	2360
			adapters
D5-15			4×8×10/7	2770
D5-16			4×8×5 vacuum tube	2360
			adapters
D5-17			4×12×5 vacuum tube	2480
D5-18			4×12×7 vacuum tube	2760
D5-19			4×10×10 vacuum tube	2880
D5-20	Microplate rotor	4000	2×3×96 well	2310
D5-21	Fixed rotor	5000	6×15ml	2540
D5-22		5000	12×15ml	3080
D5-23		5000	24×15ml	3500
D5-24		5000	30×15ml	3830
D5-25		5000	4×50ml	2520
D5-26	Fixed rotor	5000	6×50ml	2850
D5-27		4000	12×50ml	2470
D5-28		5000	4×100ml	2630
D5-29		5000	6×100ml	3130

 Adapter for 250 round cup and square cup 

VolumeCup	5ml	7ml	10ml	15ml	50ml
250ml（Square）	9pcs/cup	9	9	4	1
250ml（Round）	7pcs/cup	7	7	4	1

  Rotor with adapter

Overview

• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• ​Versatile sample input ranges
• Isolate all sizes of free-circulating RNA, including microRNA
• The purified exosomal RNA is free from any circulating RNA-binding proteins
• No phenol extractions, Proteinase K treatment, nor carrier RNA
• No time-consuming ultracentrifugation, filtration nor special syringes are required
• No precipitation reagents, nor overnight incubation required
• Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL.

Details

Supporting Data

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Kit Specifications
Minimum Plasma Input	4 mL
Maximum Plasma Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50 – 100 µL
Time to Complete 10 Purifications	40 – 45 minutes
Average Yields*	Variable depending on specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component	Cat. 59500 (50 preps)	Cat. 59600 (25 preps)	Cat. 59700 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	8 mL	2 x 8 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	2 x 20 mL	2 x 20 mL	2 x 20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	2 x 18 mL	18 mL	18 mL
Elution Solution A	2 x 6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	100	50	30
Collection Tubes	100	50	30
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples
Sample amount	Solid tissue: 1-10mg; Anticoagulant blood: 200μl
Elution volume	≥20μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability- handle a variety of liquid samples

Kit Contents

Contents	D301802	D301803
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	100	500
Buffer ATL	30 ml	150 ml
Buffer AL	30 ml	150 ml
Buffer GW1	22 ml	88 ml
Buffer GW2	12 ml	50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.