
Description
Specifications
Clone | IHC400 |
Source | Mouse Monoclonal |
Positive Control | Liver |
Dilution Range | 1:200 |
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes which plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumors.
Clone | IHC400 |
Source | Mouse Monoclonal |
Positive Control | Liver |
Dilution Range | 1:200 |
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
Features | Specifications |
Main Functions | IVD5412 precast kit for MagMix 32, smart 32 |
Applications | RT-PCR,PCR,NGS |
Products | Viral DNA / RNA, body cell DNA / RNA |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technolog |
Process method | Manual or automatic |
Sample type | |
Sample amount | 200μl |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
Cat.No | Reagent | IVD5412-TL-96 |
PK/Carrier RNA | 24mg/310μg | |
Protease Dissolve Buffer Blue | 1.8 ml | |
Tip | 12 | |
Prepackage Plate(2.0ml Plate) | Row 1/7:500µl Buffer MLBRow 2/8:500µl Buffer MW1Row 3/9:500µl Buffer CWRow 4/10:500µl Buffer CW&MPNRow 5/11:100µl Buffer AVE | 6 |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free
Conforms to SBS and ANSI requirements
Centrifugal strength up to 3000g
Square well
50 count
Labware definition is available for immediate use in Opentron’s Labware Library
NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free
Conforms to SBS and ANSI requirements
Centrifugal strength up to 3000g
Square well
50 count
Labware definition is available for immediate use in Opentron’s Labware Library
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
---|---|---|---|---|---|
55000 | Plasma/Serum RNA Purification Mini Kit | 10 – 25 µL | 50 µL – 1 mL | 250 µL – 1 mL | 5 – 10 mL |
35300 | Total RNA Purification Micro Kit | 20 – 50 µL | 1 – 4 mL | 2 – 10 mL | 10 – 20 mL |
17200 | Total RNA Purification Kit | 50 – 100 µL | 4 – 10 mL | 11 – 30 mL | 20 – 35 mL |
Figure 1 / 13
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Kit Specifications | |
Maximum Binding Capacity Per Well | 50 μg |
Maximum Loading Volume Per Well | 500 μL |
Size of RNA Purified | All sizes, including small RNA < 200 nt |
Maximum Amount of Starting Material | |
Animal Cells | 1 x 106 cells |
Animal Tissues | 10 mg |
Blood | 100 μL |
Plasma/Serum | 150 μL |
Bacteria | 1 x 109 cells |
Yeast | 1 x 108 cells |
Fungi | 40 mg |
Plant Tissues | 40 mg |
Time to Complete 96 Purifications | 30 minutes |
Average Yields* | |
HeLa Cells (1 x 106 cells) | 15μg |
E. coli Cells (1 x 109 cells) | 50 μg |
* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
---|---|---|---|---|---|---|---|---|
Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL |
Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
Mini Spin Columns | 50 | 100 | 250 | 500 | – | – | – | – |
96-Well Isolation Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Isolation Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Adhesive Tape | – | – | – | – | 4 | 12 | 4 | 12 |
Collection Tubes | 50 | 100 | 250 | 500 | – | – | ||
96-Well Collection Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Collection Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | – | – | ||
96-Well Elution Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Elution Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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