
Description
Specifications
Clone | IHC400 |
Source | Mouse Monoclonal |
Positive Control | Liver |
Dilution Range | 1:200 |
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes which plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumors.
Clone | IHC400 |
Source | Mouse Monoclonal |
Positive Control | Liver |
Dilution Range | 1:200 |
N-(Propargyl-PEG4)-N-bis(PEG4-acid) is a branched crosslinking reagent with a propargyl group and two terminal carboxylic acids. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG4)-N-bis(PEG4-acid) is a branched crosslinking reagent with a propargyl group and two terminal carboxylic acids. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
An adapter for Cerillo’s Alto plate reader. This adapter allows the Alto plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
An adapter for Cerillo’s Alto plate reader. This adapter allows the Alto plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
Figure 1 / 3
Click for expanded view
Kit Specifications | |
---|---|
Sample Type | Fresh or frozen urine |
Sample Volume Range | 10 to 30 mL |
Minimum Elution Volume | 50 μL |
Maximum Elution Volume | 100 μL |
Time to Complete 10 Purifications | 40–50 minutes |
Size of RNA Purified | All sizes, including miRNA and small RNA (<200 nt) |
Average Yields ¥ | Variable depending on specimen |
¥Please check page 5 of the protocol for average urine yields and common RNA quantification methods.
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Mini (Cat. 72900) | Midi (Cat. 72910) | Maxi (Cat. 72920) |
---|---|---|---|
Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
Lysis Buffer A | 30 mL | 20 mL | 20 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Collection Tubes | 50 | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
EXTRAClean Mini Spin Columns | 50 | 20 | 10 |
EXTRAClean Midi Spin Columns | – | 20 | – |
EXTRAClean Maxi Spin Columns | – | – | 10 |
Product Insert | 1 | 1 | 1 |
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map