
Description
Specifications
Clone | IHC527 |
Source | Mouse Monoclonal |
Positive Control | Hodgkin’s Lymphoma |
Dilution Range | 1:200 |
Cluster of differentiation 15 (CD15) is a carbohydrate adhesion molecule. Positive staining for CD15 and negative staining for leukocyte common antigen or other B- or T-cell lineage markers helps recognize Reed Sternberg cells (RSC) in Classical Hodgkin’s Lymphoma (CHL), and distinguishes it from Hodgkin-like neoplasms. CD15 does not stain mesotheliomas and is therefore most useful for distinguishing epithelial mesothelioma from adenocarcinoma.
Clone | IHC527 |
Source | Mouse Monoclonal |
Positive Control | Hodgkin’s Lymphoma |
Dilution Range | 1:200 |
qPCR Probe 2x LyoCake Master Mix simplifies your PCR setup and can be stored at room temperature. A cool chain is not needed anymore. The Master Mix is freeze-dried and dissolves within a few seconds after addition of the included rehydration buffer.
After rehydration of the LyoCake, only primers, probes and template need to be added as the 2x Master Mix contains all components for a successful and reliable qPCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of qPCR performance allows the application of this mix in a wide range of PCR applications.
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
qPCR Probe 2x LyoCake Master Mix simplifies your PCR setup and can be stored at room temperature. A cool chain is not needed anymore. The Master Mix is freeze-dried and dissolves within a few seconds after addition of the included rehydration buffer.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 100-150mg stool samples |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Stool |
Sample amount | 100-150 mg |
Elution volume | |
Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
Contents | D636401 | D636402 | D636404 |
Purification Times | 48 Preps | 96 Preps | 400 Preps |
MagPure Particles N | 1.7 ml | 3.4 ml | 14 ml |
2ml Bead Tubes | 48 | 96 | 400 |
RNase A | 10 mg | 20 mg | 75 mg |
Proteinase K | 24 mg | 48 mg | 180 mg |
Protease Dissolve Buffer | 3 ml | 5 ml | 20 ml |
Buffer ATL | 60 ml | 110 ml | 300 ml |
PVP-10 | 1.2 g | 2.2 g | 6 g |
Buffer PCI | 50 ml | 100 ml | 300 ml |
Buffer MLE | 30 ml | 60 ml | 180 ml |
Buffer GW1* | 22 ml | 44 ml | 132 ml |
Elution Buffer | 20 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Description
FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.
Features:
Composition
FluoroDye™ DNA Fluorescent Loading Dye is stored in 6X concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.
Storage
Protected from light
-20°C for 24 months
FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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