GeneAb™ CD15/Leu-M1

Overview

• Extract total RNA, including virus & viroid RNA
• Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
• Isolate total RNA (including microRNA) without phenol
• Isolated RNA is of high quality, integrity and diversity
• Also available in 96-well format for high throughput applications
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. 

The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.  

Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications.  Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

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Kit Specifications – Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi	50 mg 1 x 106 cells 50 mg (wet weight)
Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves	60 μg 60 μg 32 μg 35 μg 30 μg
Time to Complete 10 Purifications	30 minutes

* Yield will vary depending on the type of sample processed.

* Yield will vary depending on the type of sample processed.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits

Plants

Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves

Fungi

Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima

Component	Cat. 25800 (50 preps)	Cat. 31350 (100 preps)	Cat. 25850 (250 preps)	Cat. 31900 (192 preps)
Lysis Buffer C	60 mL	1 x 30 mL, 1 x 60 mL	3 x 60 mL	2 x 60 mL
Wash Solution A	38 mL	38 mL	1 x 18 mL, 2 x 38 mL	2 x 38 mL
Elution Solution A	6 mL	6 mL	20 mL	20 mL
Filter Columns	50	100	250	–
Spin Columns	50	100	250	–
96-Well Plate	–	–	–	2
Adhesive Tape	–	–	–	4
Collection Tubes	100	200	500	–
96-Well Collection Plate	–	–	–	2
Elution Tubes (1.7 mL)	50	100	250	–
96-Well Elution Plate	–	–	–	2
Product Insert	1	1	1	1

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• 10 discrete bands from 50 bp to 500 bp
• Higher intensity reference band at 250 bp

The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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50 bp DNA Ladder (Cat# 13525) – 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	500	79
2	450	67
3	400	59
4	350	55
5	300	49
6	250	76
7	200	33
8	150	22
9	100	31
10	50	29

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Description 

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg²⁺ concentration as compared to other DNA polymerase products. 

Features

• 5’→3′ DNA polymerase activity
• 3’→5′ exonuclease (proofreading) activity 
• High reaction rate (up to 1 kb/10 seconds)
• High fidelity, 70 times higher than Taq DNA polymerase 
• Blunt end amplicons
• Thermo-stable: half-life is more than 10 hrs at 95°C 

Storage

[TF1000] SMO-HiFi™ DNA Polymerase

-20°C for 24 months

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.