GeneAb™ Cytokeratin 19

Product Description

High Sensitivity & Specificity DNA Isothermal Amplification Kit NFO 48 Tests/Kit by Amp-future

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.