Description
Specifications
| Clone | IHC019 |
| Source | Mouse Monoclonal |
| Positive Control | Bladder, Colon Carcinoma, Colon, Thyroid Carcinoma |
| Dilution Range | 1:200 |
Cytokeratin 19 (CK19) forms intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue and provides mechanical support. Anti-Cytokeratin 19 stains epithelia and epithelial malignancies such as carcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Cytokeratin 19 is a useful marker for distinguishing hepatocellular carcinoma from intrahepatic cholangiocarcinoma. This differentiation is improved when stained in combination with Cytokeratin 7, CAM5.2l, Ber-EP4/MOC31, HepPar1 and TTF1. Cytokeratin 19 staining can also be used to recognize thyroid papillary carcinomas.
| Clone | IHC019 |
| Source | Mouse Monoclonal |
| Positive Control | Bladder, Colon Carcinoma, Colon, Thyroid Carcinoma |
| Dilution Range | 1:200 |
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry (Lee et al., 2005). S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulasepositive, which forms the basis of traditional identification methodology.
Staphylococcus aureus TaqMan PCR Kit, 100 reactions
Staphylococcus aureus TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM29350 (100 preps) | Cat. TM29310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. aureus Primer & Probe Mix | 280 μL | 280 μL |
| S. aureus Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.
NanoSight® Analysis
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
Exosomal RNA Analysis
To purify exosomes and isolate exosomal RNA, choose the Plasma/serum Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA/TEM etc. before processing them for RNA isolation. Or you can use the Exosomal RNA Isolation Kit if you’ve already purified exosomes using a Norgen kit or another method. . Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.
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| Kit Specifications | |
| Plasma/Serum Input (Cat. 57400) | 50 μL – 1 mL |
| Plasma/Serum Input (Cat. 57500) | 1 mL – 4 mL |
| Plasma/Serum Input (Cat. 57600) | 4 mL – 10 mL |
| Size of Exosomes Purified | 40 nm – 150 nm |
| Elution Volume | Variable depending on the plasma/serum input volume |
| Time to Complete 10 Purifications | 15 – 30 minutes |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
| Component | Cat. 57400 (50 preps) | Cat. 57500 (25 preps) | Cat. 57600 (15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 8 mL | 8 mL | 2 x 8 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Mini Filter Spin Columns inserted into 2 mL tubes | 50 | 25 | 15 |
| Product Insert | 1 | 1 | 1 |
Attogene’s Microcystin Test Kit (Rapid – Drinking Water) can be used to detect microcystins in water to as low as 0.1ppb; highly sensitive, rapid, robust screening kit for microcystins and nodularins.
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on thresholds for microcytin-LR concentrations in drinking waters has been developed. This is guidance that allows states to take various actions.
For the rapid screening of microcystins in drinking water samples at or above 0.1 ppb. Samples requiring regulatory action should be confirmed by ELISA, HPLC, or other conventional methods.
To protect consumers from adverse health effects caused by these toxins, the World Health Organization (WHO) has proposed a provisional upper limit for Microcystin-LR of 1.0 ppb (μg/L) in drinking water.
The U.S. Environmental Protection Agency (EPA) has also established guidelines for Microcystins in drinking water:
-For children below school age, 0.3 μg/L (ppb)
-For all other age groups, 1.6 μg/L (ppb)
Screening of Microcystins in water samples at 0.1 ppb (drinking water)
Format: 10 tests (5 tests/5 controls)
Not provided: Water Sample Bottles
Run Time: 15 Minutes
Finished Drinking Water