E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from bacterial culture |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Gram negative or positive bacteria |
| Sample amount | Bacterial culture: 0.5-1.5ml |
| Elution volume | ≥60μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D513101 | D513102 | D513103 |
| Purification Times | 48 Preps | 96 Preps | 480 Preps |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
| Buffer SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Attogene has developed a set of RNase-Free Lateral Assay Buffers specifically designed for developers of Nucleic Acid-based lateral flow tests
Components
Set of 5 RNase-Free Lateral flow running buffers at specifically developed for optimizing nucleic acid based lateral flow assays. Each buffer is certified RNase-Free and tested functionally in RNA and DNA based lateral flow tests.
Features & Benefits
5 bottles of Running Buffer (10mL)
SKU: AU2035
