E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
Folic acid, is one of the B vitamins used to treat anemia caused by folic acid deficiency.
This kit is a product based on indirect-competitive ELISA, which is fast, easy, accurate and sensitive compared with common instrumental analysis.
Detection limit if folic acid in liquid samples
Highly Sensitive, rapid, robust screening kit for Folic Acid
These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.
Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.
Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.
Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.
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| Kit Specifications (Spin Column) | |
| Maximum Column Binding Capacity | 35 μg |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Amount of Starting Material | 35 μg of RNA |
| Minimum Elution Volume | 20 μL |
| Time to Complete 10 Purifications | 20 minutes |
| Average Recovery | ≥ 90% |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 23600 (50 preps) | Cat. 43200 (100 preps) | Cat. 25100 (192 preps) | Cat. 61000 (50 preps) |
|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 2 x 40 mL | 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 2 x 20 mL | 6 mL |
| Column Activation Solution | – | – | – | 30 mL |
| Micro Spin Columns | 50 | 100 | – | – |
| Micro-Elute RNA Spin Columns | – | – | – | 50 |
| 96-Well Plate | – | – | 2 | – |
| Adhesive Tape | – | – | 4 | – |
| Collection Tubes | 50 | 100 | – | 50 |
| 96-Well Collection Plate | – | – | 2 | – |
| Elution Tubes (1.7 mL) | 50 | 100 | – | 50 |
| 96-Well Elution Plate | – | – | 2 | – |
| Product Insert | 1 | 1 | 1 | 1 |
Description
The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis.
Features
Storage
-20°C for 24 months
| Component Volume Taq /RT Enzyme Mix 50 μl 2X One-Step Buffer 750 μl x 2 |
The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis.
