GeneAb™ GATA3

A custom designed PACE® Genotyping Assay, designed to your target sequence. Compatible with all PACE genotyping master mixes.

About

PACE (PCR Allelic Competitive Extension) genotyping chemistry is a homogeneous, PCR-based allele-specific technology for the analysis of DNA sequence variants, most commonly SNPs (Single Nucleotide Polymorphisms) and Indels (insertion / deletions).

PACE genotyping chemistry is comprised of two parts:

1. PACE Genotyping Assay: two allele-specific forward primers and one common, reverse primer. The allele-specific forward primers each have different 3′ terminal bases reflecting the target variant, and a unique 5’ tail sequence which is incorporated as part of the fluorescent signal mechanism.
2. PACE Genotyping Master Mix: containing all remaining components required for PCR and the generation of fluorescent signals. PACE Genotyping Master Mix contains a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals (FAM and HEX) corresponding to the assay genotypes.

When combined with sample DNA, these components create a PACE Genotyping Reaction, as illustrated in the figure below.

We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. PACE Genotyping Assays are available to purchase either Validated and Unvalidated. Validated assays require customer DNA to validate and optimise, for guaranteed performance. Unvalidated assays are designed in silico and supplied untested.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• Template DNA
• PCR-grade water
• PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

STEPS TO YOUR PACE GENOTYPING ASSAY DESIGN

1. Place your order on this page. Our support team will contact you by email.
2. Fill out an Assay Design Template form with all the information we need to process your custom PACE Genotyping Assay order. We will email you a copy of the template when we first contact you, or your can download a copy here.
3. Email your completed Assay Design Template to support@3crbio.com
4. Using the information you provide us, we will create your PACE Genotyping Assay designs, order the oligos, and send you design sequences.
5. Once we receive the oligos, we assemble the assay(s) and then ship an aliquot to you (unvalidated) or test on your DNA samples before shipping the aliquot to you (validated).

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

Template DNA

PCR-grade water

PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

Overview

• No need to immediately process samples
• Includes pre-loaded beads for effective sample homogenization by bead beating (tubes are fully compatible with commercially available bead beating systems)
• DNA preservation at room temperature over 2 years
• Ship fecal samples at room temperature safely
• Compatible with most DNA isolation methods 

Norgen’s Fecal DNA Collection & Preservation Mini Tubes are designed for the rapid preservation of DNA from fresh fecal specimens. These tubes contain Norgen’s Fecal DNA Preservative in a liquid format. Moreover, each collection tube is preloaded with beads for effective sample homogenization in any commercially available bead beater equipment (e.g. MP Biomedicals’ FastPrep®-24). The user simply collects fecal samples into the tubes and mixes by shaking or vortexing until the feces is mixed well with preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow the DNA in the samples to be preserved at room temperature for over 2 years. 

This kit is highly suited to collection and preservation of fecal material from mouse, hamster, chinchilla, rabbit and more for microbiome studies and other applications.

DNA Isolation from Preservative

Fecal samples collected and preserved in Norgen’s Fecal DNA Collection and Preservation Mini Tubes are compatible with most fecal DNA isolation methods. Samples stored in the tubes have been used successfully with all of Norgen Biotek’s stool-based DNA isolation kits (please refer to Related Products table) and reagents.  

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Details

Supporting Data

Figure 1 / 4

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Kit Specifications
Fecal Input	Maximum 30 mg (approximately 3-5 mouse droppings)
Stability of Fecal DNA at Room Temperature	2 years

Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Kit Components	Cat. 27650
Fecal DNA Collection & Preservation Mini Tubes (containing liquid preservative and beads)	12
Product Insert	1

Overview

• Extract high quality & quantity total RNA including miRNA 
• No phenol step required; isolate all RNA in one fraction
• Genomic DNA Removal Column for efficient elimination of gDNA
• Bind & elute all RNA irrespective of size or GC content, without bias
• Efficiently extract small RNA irrespective of GC content
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Isolate from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step. 

Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more.  Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.  Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Column	Up to 50 μg RNA
Maximum Loading Volume Per Spin Column	650 μL
Size of RNA Purified	All sizes, including < 200 nt
Time to Complete 10 Purifications	25 minutes
Maximum Amount of Starting Material
Liver Tissue	20 mg
Heart Tissue	5 mg
Kidney Tissue	20 mg
Brain Tissue	25 mg
Lung Tissue	20 mg
Spleen Tissue	20 mg
Whole Blood	100 µL
Hela Cells	3 x 106
CHO	3 x 106
Yeast	1 x 108 cells
E.coli	1 x 109 CFU/mL
Bacteria	1 x 109 cells
Plasma/Serum	200 µL
Fungi	50 mg
Plant Tissue	50 mg
Viruses	≤100 µL of viral suspension
Average Yield
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48300 (50 preps)	Cat. 48400 (100 preps)
Buffer RL	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 6 mL
RNA Purification Columns	50	100
Genomic DNA Removal Columns	50	100
Collection Tubes	100	200
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1