Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications	PCR, southern bolt and virus detection, etc
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• High throughput – 96 samples can be processed simultaneously

Kit Contents

Contents	D311701	D311702
Purification Times	1 x 96	4 x 96
HiPure gDNA Plate	1	4
96 well Plate (2.2ml)	1	4
1.6ml Collection Plate	1	4
0.5ml Collection Plate	1	4
Silicon Seal Tape	1	4
Seal Film	5	25
Buffer ATL	30 ml	100 ml
Buffer AL	30 ml	100 ml
Buffer DW1	60 ml	250 ml
Buffer GW2	50 ml	2 x 100 ml
Proteinase K	50 ml	200 ml
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	30 ml	120 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Sample amount	Leukocyte protocol*	Colum type	Elutio volume	Average yield	Time per run
HiPure Blood DNA Mini Kit	D3111	10-200μl	1ml	2ml column	≥20μl	5-9μg/200μl	≤30 minutes
HiPure Tissue&Blood DNA Midi Kit	D3113	0.2-2ml	10ml	1.5ml column	≥300μl	20-40μg/1m	≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit	D3115	3 -10ml	10ml	15ml column	≥700μl	20-40μg/1m	≤90 minutes
HiPure Tissue&Blood DNA 96 Kit	D3117	1-200μl	1ml	96 well plate		3-8μg/200μl

*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Cell Culture Dish 35mm

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

• Gamma radiation sterilization
• Non-Pyrogenic, DNase/Rnase free.

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm