
Description
Specifications
Clone | IHC583 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma, Urothelial Carcinoma |
Dilution Range | 1:200 |
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and Her2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumors, therefore Anti-GATA3 is useful for carcinoma diagnosis when breast and bladder are plausible.
Clone | IHC583 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma, Urothelial Carcinoma |
Dilution Range | 1:200 |
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from FFPE tissue |
Applications | RT-PCR, cDNA synthesis, second generation sequencing |
Products | RNA, miRNA |
Purification method | Mini spin column |
Purification technology | Silica technology, DNase |
Process method | Manual (centrifugation or vacuum) |
Sample type | FFPE slice, FFPE embedded tissue |
Sample amount | Tissue: <6 mgFFPE: <6 slices |
Yield | 1-20μg |
Elution volume | ≥15μl |
Time per run | ≤30 minutes (after digestion) |
Liquid carrying volume per column | 800μl |
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA wasfinally eluted with low-salt buffer.
Advantages
Kit Contents
Contents | IVD4144 |
Purification Times | 50 Preps |
HiPure RNA Micro Columns | 50 |
2ml Collection Tubes | 50 |
Proteinase K | 24 mg |
Protease Dissolve Buffer | 1.8 ml |
DNase I | 600 μl |
DNase Booster Buffer | 1.5 ml |
Buffer DPS | 60 ml |
Buffer FRL | 15 ml |
Buffer RLC | 15 ml |
Buffer RWC* | 10 ml |
Buffer RW2* | 20 ml |
RNase Free Water | 10 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
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