GeneAb™ GATA3

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments
• Double intensity reference bands at 200 bp and 400 bp

The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.

Contents:

1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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MiniSizer 50 bp DNA Ladder (Cat# 11200) – 100 loads

Ladder Properties:
• Fourteen discrete bands, ranging from 50 bp to 650 bp
• Higher intensity bands at 200 bp and 400 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	650	61
2	600	46
3	550	36
4	500	30
5	450	35
6	400	85
7	350	27
8	300	25
9	250	16
10	200	54
11	150	33
12	100	21
13	50	17
14	25	14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.

DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.

The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies. 

SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot 

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

RLE500R

Features

• SBS/ SLAS footprint
• Integrated cushion base for precise pipetting
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated bracket to help keep microplate in place and flat
• Made in USA 
• For Research use only

Compatibility

• Full- skirt PCR plates
• 1/2- skirt PCR plates 
• Un-skirted PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. SPTLabtech Firefly®, Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 150 µL 

Minimum  – 5 µL 

The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.