
Description
Specifications
Clone | IHC583 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma, Urothelial Carcinoma |
Dilution Range | 1:200 |
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and Her2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumors, therefore Anti-GATA3 is useful for carcinoma diagnosis when breast and bladder are plausible.
Clone | IHC583 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma, Urothelial Carcinoma |
Dilution Range | 1:200 |
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
Specifications
Features | Specifications |
Main Functions | Isolation bacterial DNA from cultures, food and other samples |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Culture medium, swab, parasitic blood, tissue,sputum, etc. |
Sample amount | Bacterial culture medium: 0.5-2 mlTissue samples: 50-100 mg Whole blood / cell suspension: 0.5-1 ml Viscous secretion: 0.1-1 g |
Elution volume | ≥30μl |
Time per run | ≤40 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.
Kit Contents
Contents | D314602 | D314603 |
Purification Times | 50 Preps | 250 Preps |
Hipure DNA Mini Columns I | 50 | 2 x 125 |
2ml Collection Tubes | 50 | 2 x 125 |
Glass Beads (0.1~0.2mm) | 20 g | 100 g |
Buffer P1 | 20 ml | 100 ml |
Buffer DL | 15 ml | 80 ml |
Buffer GW1 | 22 ml | 88 ml |
Buffer GW2 | 12 ml | 50 ml |
Lysozyme | 60 mg | 300 mg |
Proteinase K | 24 mg | 120 mg |
Protease Dissolve Buffer | 5 ml | 15 ml |
Buffer AE | 15 ml | 60 ml |
Storage and Stability
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.
Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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