Description 

AccuBand™ 100 bp+3K DNA Ladder II is composed of 14 individual DNA fragments, presenting 3k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700 600, 500, 400, 300, 200 and 100 bp sharp bands respectively. This product contains 2 enhanced bands (1k and 500 bp) for easy band identification. AccuBand™ 100 bp+3K DNA Ladder II is ready-to-use, containing loading buffer with dual color tracking dyes (orange G and Xylene cyanol FF). AccuBand™ 100 bp+3K DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 100 bp to 3 kb, either in agarose gel or in polyacrylamide gel electrophoresis.

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 3,000 bp 

Concentration 

54.4 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

Plasmid Purification Magnetic Beads (RNA Depletion)

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step.  Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.

Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc.  The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.

Features

• Effective depletion of bacterial RNA by RNase
• High recovery rate of plasmid DNA by magnetic beads
• Removal of unwanted components and impurities
• Simple and fast beads-based protocol

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.