
Description
Specifications
Clone | IHC132 |
Source | Mouse Monoclonal |
Positive Control | Astrocytoma |
Dilution Range | 1:200 |
Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.
Clone | IHC132 |
Source | Mouse Monoclonal |
Positive Control | Astrocytoma |
Dilution Range | 1:200 |
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from bacteria culture |
Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Bacteria culture |
Sample amount | Bacteria: <10^9 |
Elution volume | ≥30μl |
Time per run | ≤40 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100µg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
Contents | R418101 | R418102 | R418103 |
Purification Times | 10 Preps | 50 Preps | 250 Preps |
gDNA Filter Mini Columns | 10 | 50 | 250 |
HiPure RNA Mini Columns | 10 | 50 | 250 |
2ml Collection Tubes | 20 | 100 | 500 |
Glass Beads (0.1-0.6mm) | 10 g | 30 g | 150 g |
Plastic spoon | 2 | 4 | 10 |
Lysozyme | 20 mg | 90 mg | 400 mg |
Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 10 ml |
Buffer TE | 1.8 ml | 1.8 ml | 5 ml |
Buffer STL | 5 ml | 20 ml | 90 ml |
Buffer RLC | 10 ml | 30 ml | 150 ml |
Buffer RW1 | 10 ml | 50 ml | 250 ml |
Buffer RW2* | 5 ml | 20 ml | 2 x 50 ml |
RNase Free Water | 1.8 ml | 10 ml | 30 ml |
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Figure 1 / 1
Click for expanded view
Ladder Properties:
• 22 discrete bands, ranging from 60 bp to 600 bp
Storage:
• Store at -20°C in small aliquots. For longer term storage, -70°C is recommended.
• The product is light-sensitive. Minimize light exposure.
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