GeneAb™ LMO2

The Multiplexing Index Primers contain primer mix for multiplexing library samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS library samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.

With library multiplexing, unique index sequence is added to individual sample during NGS library preparation.  Therefore, each DNA molecule can be identified after pooling of multiple samples based on the index information they have.

Each of our index primers contains a unique index sequence with 6 bases that can be used to identify libraries. Library multiplexing up to 48 samples is possible.

Multiplexing Index Primers (illumina platform): Even distribution of 48 samples using index primers. 48 libraries were made using the BioDynami NGS DNA Library Prep Kit (Cat. # 30009) and the BioDynami Multiplexing Index Primers (Cat. # 30072). Libraries were pooled at equal concentration and sequenced on the illumina HiSeq 2500. The numbers of reads from 48 libraries were analyzed.

List of index sequence for the primers (each of the index primer mix contains universal primer and one of the index primers). Index number and the index sequence are listed.

List of indexes can be downloaded Here.

Sequence of the final library with index location:

The Multiplexing Index Primers contain primer mix for multiplexing library samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS library samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.

Aminooxy-PEG1-propargyl is a click chemistry PEG linker containing a propargyl group and an aminooxy group. The aminooxy group is reactive with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.

Aminooxy-PEG1-propargyl is a click chemistry PEG linker containing a propargyl group and an aminooxy group. The aminooxy group is reactive with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.