
Description
Specifications
Clone | IHC409 |
Source | Mouse Monoclonal |
Positive Control | Colon, Colon Carcinoma |
Dilution Range | 1:200 |
MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumors that are microsatellite-unstable.
Clone | IHC409 |
Source | Mouse Monoclonal |
Positive Control | Colon, Colon Carcinoma |
Dilution Range | 1:200 |
This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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Kit Specifications – Spin Column | |
Column Binding Capacity | 10 μg |
Size of DNA Purified | 100 – 15,000 bp |
Average Recovery | > 90% |
Average Primer Removal | > 90% |
Minimum Elution Volume | 30 μL |
Time to Complete 10 Purifications | 15 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
---|---|---|---|
Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
Spin Columns | 50 | 250 | – |
Collection Tubes | 50 | 250 | – |
96-Well Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
96-Well Collection Plate | – | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – |
96-Well Elution Plate | – | – | 2 |
Product Insert | 1 | 1 | 2 |
029070 Nitrate Reduction Reagent
Usage:For Nitrate Reduction Test.
Specification:10ml*2vials
10ml*2vials
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