
Description
Specifications
Clone | IHC409 |
Source | Mouse Monoclonal |
Positive Control | Colon, Colon Carcinoma |
Dilution Range | 1:200 |
MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumors that are microsatellite-unstable.
Clone | IHC409 |
Source | Mouse Monoclonal |
Positive Control | Colon, Colon Carcinoma |
Dilution Range | 1:200 |
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 5ml plasma, serum, body fluids |
Applications | qPCR, NGS, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Serum, plasma |
Sample amount | 5ml |
Elution volume | ≥40μl |
Time per run | ≤50 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Contents | 1292750 | 12927200 |
Purification Times | 50 | 200 |
MagPure Particles G | 20 ml | 80 ml |
MagBind Particles (selection particles) | 14 ml | 58 ml |
Selection Solution | 100 ml | 400 ml |
Proteinase K | 300 mg | 1.2 g |
Protease Dissolve Buffer | 25 ml | 100 ml |
Buffer SDS(20%) | 15 ml | 60 ml |
Buffer MLK | 300 ml | 3 x 450 ml |
Buffer BST1 | 225 ml | 2x 450 ml |
Buffer MKW1 | 225 ml | 2x 450 ml |
Buffer MW2* | 50 ml | 2x 100 ml |
Buffer AE | 10 ml | 30 ml |
Storage and Stability
MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at roomtemperature (15–25°C) and are stable for at least 18 months underthese conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.
Contents | D635901D | D635902D | D635903D |
Purification Times | 48 | 96 | 480 |
MagPure Particles N | 1.1 ml | 2.2 ml | 11 ml |
Buffer ATL | 40 ml | 80 ml | 2 x 200 ml |
Buffer BGL | 40 ml | 80 ml | 2 x 200 ml |
Buffer BST1 | 60 ml | 120 ml | 550 ml |
Buffer GW2* | 20 ml | 25 ml | 2 x 50 ml |
Proteinase K | 48 mg | 110 mg | 2 x 220 mg |
Protease Dissolve Buffer | 5 ml | 10 ml | 30 ml |
DTT | 235 mg | 235 mg | 3 x 235 mg |
Elution Buffer | 10 ml | 10 ml | 30 ml |
32/48 channel machine
Product | Contents and volume | D6359D-TL-06 |
Buffer BGL | 80 ml | |
Buffer ATL | 80 ml | |
Proteinase K | 110 mg | |
Protease Dissolve Buffer | 10 ml | |
DTT Powder | 2 x 235 mg | |
Elution Buffer | 5 ml | |
AS-Tip | 12 pcs | |
2.0ml V bottom plate | Row 1/7:empty | 6 plates |
Row 2/8:500μl Buffer BST1 | ||
Row 3/9:500μl Buffer BST1 | ||
Row 4/10:20μl MagPure Particles N 500μl Buffer GW2 Row 5/11:500μl Buffer GW2 (ethanol ~350ul) | ||
Row 6/12:60μl Elution Buffer |
96 channel machine
Product | Contents and volume | D6359D-F-96 | D6359D-F-48 |
Buffer BGL | 80 ml | 40 ml | |
Buffer ATL | 80 ml | 40 ml | |
Proteinase K | 110 mg | 48 mg | |
Protease Dissolve Buffer | 10 ml | 5 ml | |
DTT Powder | 235 mg | 235 mg | |
Elution Buffer | 5 ml | 5 ml | |
96-Tip (AS) | 1 | 1 | |
Sample Plate 1 | 500μl Buffer BST1 | 1 | 1 |
Sample Plate 2 | 500μl Buffer BST1 | 1 | 1 |
Wash Plate 1 | 20μl MagPure Particles N500μl Buffer GW2 | 1 | 1 |
Wash Plate 2 | 500μl Buffer GW2 | 1 | 1 |
Elute Plate | 60μl Elution Buffer | 1 | 1 |
Storage and Stability
Proteinase K and DTT Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.
DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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