Description
Specifications
| Clone | IHC410 |
| Source | Mouse Monoclonal |
| Positive Control | Colon, Colon Carcinoma |
| Dilution Range | 1:200 |
MutS Homolog 2 (MSH2) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Expression levels of MSH2 are abnormally low in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MSH2 is optimized when paired in an IHC panel with antibodies against MSH6, MLH1, and PMS2. Reports have shown Anti-MSH2 to be useful in the detection of the protein in a number of normal and neoplastic tissues, and for identifying a loss of MSH2 in tumors that are microsatellite-unstable.
| Clone | IHC410 |
| Source | Mouse Monoclonal |
| Positive Control | Colon, Colon Carcinoma |
| Dilution Range | 1:200 |
Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
Giardia intestinalis TaqMan RT-PCR Kit, 24 reactions
Figure 1 / 3
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Storage Conditions
The Giardia intestinalis TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. DxTM43800 (24 rxns) |
|---|---|
| MDx TaqMan 2X RT-PCR Master Mix Dx | 550 µL |
| Giardia intestinalis Primer & Probe Mix Dx | 2 x 70 µL |
| Giardia intestinalis Positive Control Dx – 200,000 copies/μL | 50 µL |
| Nuclease-Free Water | 2 x 1.25 mL |
| Product Insert | 1 |
Product Details
Sample Type:
Serum, Plasma, Whole Blood
Package Size:
48 Tests/Kit
Test Time:
5-20 Minutes
Target Disease:
African Swine Fever Virus
Product Name:
African Swine Fever Virus Isothermal Detetction Fluorescence Kit
Test Principle:
Nucleic Acid Detection
Target Species:
Pigs
High Light:
,
,
Product Description
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
| Kit composition (WLRE8208KIT 48T/Pack) | |
| Composition | Content |
| E buffer | 1 mL×2 Tubes |
| B buffer | 150 μL×1Tube |
| Positive control template | 100 μL×1Tube |
| Reagents | 48T |
Product features and advantages:
| Product features and advantages | |
| Features | Advantages |
| High sensitivity | 1 copies/ml |
| Strong specificity | Specificity is superior to PCR |
| Short reaction time | 20min |
| Polymorphic reagent | Liquid, freeze-dried powder, freeze-dried ball |
| Easy to operate | Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple |
| Easy to store and transport | It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation |
| Low requirements on equipment | Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector |
Product application:
| Application | |
| Ultra-clean laboratory | African Swine Fever Virus Isothermal Detection |
| Indoor | |
Operation procedure and process:
| Operation procedure and process: |
| Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix. |
| Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent. |
| Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid |
| Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control |
| Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap) |
| Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone. |
Performence Comparison Data:
| No. | TT value | Template concentration | Result determination |
| 1 | 4.2 | 10-1 | Positive |
| 2 | 5.5 | 10-2 | Positive |
| 3 | 10.2 | 10-3 | Positive |
| 4 | 17.0 | 10-4 | Positive |
| 5 | 12.0 | 10-5 | Positive |
| 6 | – | 10-6 | Negative |
| 7 | – | 10-7 | Negative |
| 8 | Negative control | Negative | |
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
| Clone | IHC010 |
| Source | Mouse Monoclonal |
| Positive Control | Melanoma, Skin Melanocytes |
| Dilution Range | 1:200 |
SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as transcription factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurofibromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg-Shah and Waardenburg-Hirschsprung disease. Anti-SOX-10 has been shown to be sensitive for conventional, spindled, and desmoplastic melanoma, and has been used to detect metastatic melanoma and nodal capsular nevus in sentinel lymph nodes.