Description
Specifications
| Clone | IHC026 |
| Source | Rabbit Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
| Clone | IHC026 |
| Source | Rabbit Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
K-CERA
SKU: 700004273
100 assays per kit
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.003 U/g |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 30 min |
| Application examples: | Cereal flours, fermentation broths and other materials. |
| Method recognition: | AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018) |
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
Browse the complete list of our enzyme activity assay kits.
Advantages
Validation of Methods
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from FFPE using high bind beads |
| Applications | RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Large quantities of solids |
| Sample amount | Appropriate |
| Elution volume | ≥50μl |
| Time per run | 30 – 120 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
1.Use non-toxic dewaxing solution without contact with xylene
2.Efficient removal of formaldehyde modification on DNA and improvement of PCR sensitivity
3. One of the best FFPE DNA extraction kits on the market, the same effect of paraffin slice extraction as top brand, and the effect of puncture sample extraction is even better than top brands
| Contents | D632301B | D632302B |
| Purification Times | 48 Preps | 96 Preps |
| MagBind Particles | 1.1 ml | 2 x 1.1 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer AL | 15 ml | 30 ml |
| Buffer BD* | 6 ml | 15 ml |
| Buffer BXW1* | 13 ml | 44 ml |
| Elution Buffer | 15 ml | 30 ml |
Storage and Stability
RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
The future of lab automation is here.
Opentrons Flex is a liquid-handling robot designed for high throughput and complex workflows. The Flex robot is the base of a modular system that includes pipettes, a labware gripper, on-deck modules, and labware — all of which you can swap out yourself. Flex is designed with a touchscreen so you can work with it directly at the lab bench, or you can control it from across your lab with the Opentrons App or our open-source APIs.
The future of lab automation is here.
Opentrons Flex is a liquid-handling robot designed for high throughput and complex workflows. The Flex robot is the base of a modular system that includes pipettes, a labware gripper, on-deck modules, and labware — all of which you can swap out yourself. Flex is designed with a touchscreen so you can work with it directly at the lab bench, or you can control it from across your lab with the Opentrons App or our open-source APIs.