
Description
Specifications
Clone | IHC026 |
Source | Rabbit Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
Clone | IHC026 |
Source | Rabbit Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.
Step into the future of high-throughput PCR genotyping with PACE Nano! Backed by decades of expertise in high-throughput genotyping reagent manufacturing, our groundbreaking product is engineered specifically to excel in low and ultra-low volume reactions. Say goodbye to performance variability as PACE Nano Genotyping Master Mix and PACE Genotyping Assays raise the bar for reliability and performance in the field.
With superior cost-effectiveness and versatile compatibility, PACE Nano Genotyping Master Mix ensures consistent results across diverse platforms, samples, and markers, including 384- and 1536-well PCR, Array Tape™, SNPline™ and Gene Mathematica from HC Scientific, available from 3CR Bioscience, supporting reaction volumes as low as 0.8 µL. Join the revolution today and elevate your research with PACE Nano.
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
Clone | IHC531 |
Source | Mouse Monoclonal |
Positive Control | Tonsil |
Dilution Range | 1:200 |
Cluster of differentiation 2 (CD2) is a useful early T-cell lineage restricted antigen that is present in T-cell differentiation. As a pan-T-cell marker, CD2 staining is used for recognizing practically all normal T-cells, but may be deleted in some T-cell neoplasms. Since CD2 is present in most precursor and mature T-cell leukemias and lymphomas, it is useful in the evaluation of lymphoid malignancies. By using CD2 and CD25 staining, the recognition of systemic mastocytosis and mastocytic leukemia is supported.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from <10 mg insect tissue |
Applications | PCR, southern bolt and virus detection, etc |
Purification method | Midi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Insect tissue samples |
Sample amount | <10 mg |
Elution volume | ≥15μl |
Time per run | ≤30 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Contents | D312902 | D312903 |
Purification Times | 50 | 250 |
HiPure DNA Mini Columns I | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
Buffer ITL | 30 ml | 120 ml |
Buffer IL* | 30 ml | 120 ml |
Buffer GW1* | 22 ml | 110 ml |
Buffer GW2* | 20 ml | 2 x 50 ml |
Proteinase K | 24 mg | 120 mg |
Protease Dissolve Buffer | 1.8 ml | 15 ml |
Buffer AE | 15 ml | 60 ml |
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
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