
Description
Specifications
Clone | IHC026 |
Source | Rabbit Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
Clone | IHC026 |
Source | Rabbit Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.
Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.
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Kit Specifications | |
Maximum Input | 2 x 109 bacterial cells |
Column Binding Capacity | 25 μg |
Average Yield | up to 20 µg |
Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 67900 (50 preps) |
---|---|
Precipitation Solution B | 15 mL |
Lysis Buffer T | 6.5 mL |
Solution BX | 28 mL |
Wash Solution A | 38 mL |
Elution Buffer B | 8 mL |
Micro Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Norgen’s Clostridium difficile TaqMan PCR Lyophilized Kit is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Clostridium difficile TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
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Component | Cat. TM37150L (100 preps) | Cat. TM37110L (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | – |
Clostridium difficile Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
Clostridium difficile Positive Control (Lyo) | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
Product Insert | 1 | 1 |
Strong adhesive seal with cross-cut windows. This seal is peelable and suitable for 96 well plates (auto samplers, HPLC, sequencers).
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