
Description
Specifications
Clone | IHC026 |
Source | Rabbit Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
Clone | IHC026 |
Source | Rabbit Monoclonal |
Positive Control | Colon Cancer |
Dilution Range | 1:200 |
Formaldehyde is an organic compound with the formula CH2O. It is mainly used in the production of industrial resins but has been found to be used as a preservative, disinfectant and biocide. Because of its toxicity and volatility formaldehyde poses a significant risk to human health. Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
Measuring range / color- Number of scale graduation | Number of determinations |
0.1 – 0.25 – 0.4 – 0.6 – 0.8 – 1.0 – 1.5 mg/l HCHO | 100 |
Formaldehyde is found in:
It is a byproduct of combustion and certain other natural processes, and so is also found in:
White Paper from Nix Color Sensor using Attogene’s Formaldehyde Kit: Nix Color Sensor & Attogene Formaldehyde Kit
Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
Figure 1 / 2
Click for expanded view
Kit Specifications | |
Maximum DNA Input | 25 μg |
Final Endotoxin Level | ≤ 0.1 EU/µg DNA |
Size of DNA Purified | Up to 13,000 bp |
Maximum DNA Volume Input | 100 μL |
Average Recovery | > 90% |
Time to Complete 10 Purifications | 20 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
---|---|---|---|
Buffer SK | 15 mL | 60 mL | 60 mL |
Wash Solution H | 18 mL | 18 mL | 18 mL |
Elution Buffer I | 6 mL | 12 mL | 12 mL |
Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
Precipitation Solution | – | 1.5 mL | 1.5 mL |
Spin Columns (assembled with Collection Tubes) | – | 10 | 4 |
Spin Columns | 25 | – | – |
Collection Tubes | 25 | – | – |
Elution Tubes (1.7 mL) | 25 | – | – |
Elution Tubes (15 mL) | – | 10 | – |
Elution Tubes (50 mL) | – | – | 4 |
Product Insert | 1 | 1 | 1 |
Permagen’s direct replacement magnet plate for either PN: A32782 & A001322. Comes with spring cushion base and same strength magnets to meet current protocol requirements. 49% Cost savings*
For faster separations you can upgrade to our SKU# MSP500R which comes with all of the same features, just stronger magnets.
Compatible with any magnetic beads & all protocols requiring a 96S super magnet plate
A092722
Maximum – 2 mL
Minimum – 30 µL
Permagen’s direct replacement magnet plate for either PN: A32782 & A001322. Comes with spring cushion base and same strength magnets to meet current protocol requirements. 49% Cost savings*
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map