GeneAb™ MSH6

Boc-Gly-PEG3-endo-BCN is a Boc protected cick chemistry reagent with a BCN group. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group is reactive with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Boc-Gly-PEG3-endo-BCN is a Boc protected cick chemistry reagent with a BCN group. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group is reactive with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-CellG5-4V

SKU: 700004272

The K-CellG5-2V pack size has been discontinued (read more).

Cellulase Activity Assay Kit.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

View Cellulase Activity Assay Protocol.

View our complete list of assay kits for enzyme activities.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.

About

ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.

ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.

Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.