Description 

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels. 

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems. 

Key Features

• User-friendly gel cassette:
  • Numbered and framed wells for sample loading
  • Labeled warning sign and green tape as reminder
• Enhanced gel performance: 
  • Enhanced band sharpness
  • Better resolution of small proteins 
  • Stable for shipping at ambient temperature
• Easy compatibility: 
  • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
  • Compatible with most popular protein electrophoresis systems 

Storage and stability

Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis. 

Keep Q-PAGE™ Precast Gels flat during storage.

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Name of Product

Aspergillus Antigen
(Galactomannan) – ELISA

Catalog Number

ASPE-096

Short Info

This ELISA kit is for the quantitative detection of specific Aspergillus
Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).

This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format, TMB, λ=450 nm

Range/Assay Sensivity

Limit of Detection (LOD): 0,5 ng/ml
Limit of Quantification (LOQ): 1,0 ng/ml

Test Principle

FungaDia Aspergillus Galactomannan ELISA Detection Kit is a one-step enzyme sandwich microplate immunoassay which
detects galactomannan in human serum and BAL fluid.
Galactomannan antigen in the sample bind to anti-galactomannan antibodies (sensitized on the microtiter plates) and conjugate (monoclonal antibody/peroxidase). The presence of Galactomannan Antigen is detected with substrate TMB.

12 x 8 microwell plate (96 tests)

This ELISA kit is for the quantitative detection of specific Aspergillus
Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).

This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Introduction

This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from tissue and cells and plant
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue
Sample amount	Cells: 1 x 107Animal tissue: ≤20mgPlant tissue: ≤100mgYeast cell: ≤5 x 106
Yield	5-100μg

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.

Advantages

• High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – extraction without manual participation, saving time and effort
• Universal – suitable for various animal tissues, cultured cells and conventional plant fungal tissues

Kit Contents

Contents	R662201	R662202	R662203
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure RNA Particles	1.7 ml	4.0 ml	18 ml
Proteinase K	24 mg	50 mg	240 mg
Protease Dissolve Buffer	1.8 ml	5 ml	15 ml
DNase I	600 μl	2 x 600 μl	10 x 600 μl
DNase Buffer	30 ml	40 ml	200 ml
RTL Lysis Buffer	40 ml	80 ml	400 ml
Buffer MCB*	18 ml	30 ml	150 ml
Buffer MW1*	44 ml	66 ml	2 x 220 ml
Buffer RW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	30 ml	120 ml

Storage and Stability

MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.

This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.