
Description
Specifications
Clone | IHC623 |
Source | Mouse Monoclonal |
Positive Control | Breast, Colon, Associated Adenocarcinomas |
Dilution Range | 1:200 |
Mucin 1 (MUC1) is a membrane-bound glycoprotein involved in a number of protective and cell-signaling functions, including cell-cell adhesion, proliferation, motility, invasion, and survival. Overexpression of MUC1 is clinically indicated in breast carcinomas, papillary thyroid carcinomas, and thymic carcinomas, and reports have named MUC1 as a useful marker for differentiating thymic carcinoma from type B3 thymoma. The expression of MUC1 is correlated with the grade of malignancy in thymic epithelial tumors, and loss of MUC1 expression has been associated with reactive gastropathy. MUC1 is not expressed in normal human epidermis, but has been detected in the epidermis of psoriatic plaques of biopsies from patients diagnosed with psoriasis vulgaris.
Clone | IHC623 |
Source | Mouse Monoclonal |
Positive Control | Breast, Colon, Associated Adenocarcinomas |
Dilution Range | 1:200 |
*Optical system: equipped with infinity plan achromatic stress-free metallographic objective lens,built-in polarizing ober-vation device
*Observation head device:The lens barrel can be rotated 360 degrees,and the interpupillary distance adjustment range is 50-75MM
*Eyepiece configuration:random eyepiece WF10X /22mm;
*Objective:Infinity plan achromatic strain-free metallographic objective;5X,10X,20X,50X
*Loading platform:Stage:Tripod XY composite mechanical moving stage; size 140X120MM, stroke 80X50MM, minimum reading value 0.1mm
Specification configuration | XTL-20A | Trinocular observation head,transmission single light source 5W LED ighting |
XTL-20B | Trinocular observation head,transparent reflection double light source up and down 5W LED lighting | |
Eyepiece | Wide-angle eyepiece WF10Xfield of view 22mm,optional WF16X,WF25X | |
Converter | Quadruple Revolving converter | |
Objective | Infinite field achromatic stress-free objective,5X,10X,20X,50X | |
Condenser | N.A.1.25 Swinging Abbe condenser with variable light bar | |
Stage | Double Layer Mechanical Stage,Size1140X120mn,Moving Range :80x50mm | |
Focusing | Coaxial Coase &Fine adjustment,fine division 0.02mm | |
Light source | LED 5V lighting,DC1272A power adapter,adjustable brightness | |
Attachments | Dust bag,Type C power cord |
XTL-20 series metallographic microscopes are equipped with infinity plan achromatic stress-free metallographic objective lens and Wide-angle eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Model:
XTL-20
Brand:
Yingtai
Sample type purification kit guide
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Figure 1 / 3
Click for expanded view
Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Step | Component | Cat. 70200 (24 preps) | Cat. 70210, 70220, 70230, 70240 (96 preps) |
---|---|---|---|
Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
16S V1-V3 Primer Mix | 70 µL | 280 µL | |
Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
N7xx Index Primer | 50 µL | 50 µL | |
S5xx Index Primer | 70 µL | 70 µL | |
PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
Specifications
Features | Specifications |
Main Functions | Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture |
Applications | Cell transfection, animal injection, etc. |
Purification method | Maxi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Conventional plasmid vector |
Sample amount | High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium |
Yield | 200-1500μg |
Elution volume | ≥0.7ml |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 20ml |
Binding yield of column | 1mg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P115602 | P115603 |
Purification Times | 10 Preps | 50 Preps |
RNase A | 30 mg | 150 mg |
Buffer P1 | 100 ml | 500 ml |
Buffer P2 | 100 ml | 500 ml |
Buffer LN3 | 50 ml | 250 ml |
Buffer PW1 | 33 ml | 180 ml |
Buffer PW2 | 20 ml | 100 ml |
Elution Buffer | 15 ml | 120 ml |
Buffer CL | 33 ml | 180 ml |
HiPure DNA Maxi Columns C | 10 | 50 |
Lysate Clear Midi Syringe | 10 | 50 |
50 ml Collection Tubes C | 20 | 100 |
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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