
Description
Specifications
Clone | IHC625 |
Source | Mouse Monoclonal |
Positive Control | Stomach |
Dilution Range | 1:200 |
Mucin 5AC (MUC5AC) is a secretory-type mucin found in columnar mucous cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon, but not in normal colon cells. MUC5AC expression is indicated in carcinomas wherein the type is defined as diffuse and infiltrative, and those located mainly in the antrum. Studies have also suggested a correlation between MUC5AC and colorectal signet-ring cell carcinoma, with overexpression of MUC5AC relating to the carcinogenesis, malignant potential, progression, and clinical behaviors.
Clone | IHC625 |
Source | Mouse Monoclonal |
Positive Control | Stomach |
Dilution Range | 1:200 |
These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.
Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.
Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.
Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.
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Kit Specifications | |
Maximum Column Binding Capacity | 10 μg |
Size of RNA Purified | All sizes, including small RNA (<200 nt) |
Maximum Amount of Starting Material | 10 μg of RNA |
Minimum Elution Volume | 8 μL |
Time to Complete 10 Purifications | 20 minutes |
Average Recovery | ≥ 90% |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 23600 (50 preps) | Cat. 43200 (100 preps) | Cat. 25100 (192 preps) | Cat. 61000 (50 preps) |
---|---|---|---|---|
Buffer RL | 40 mL | 2 x 40 mL | 2 x 40 mL | 40 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | 38 mL |
Elution Solution A | 6 mL | 2 x 6 mL | 2 x 20 mL | 6 mL |
Column Activation Solution | – | – | – | 30 mL |
Micro Spin Columns | 50 | 100 | – | – |
Micro-Elute RNA Spin Columns | – | – | – | 50 |
96-Well Plate | – | – | 2 | – |
Adhesive Tape | – | – | 4 | – |
Collection Tubes | 50 | 100 | – | 50 |
96-Well Collection Plate | – | – | 2 | – |
Elution Tubes (1.7 mL) | 50 | 100 | – | 50 |
96-Well Elution Plate | – | – | 2 | – |
Product Insert | 1 | 1 | 1 | 1 |
Clone | IHC010 |
Source | Mouse Monoclonal |
Positive Control | Melanoma, Skin Melanocytes |
Dilution Range | 1:200 |
SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as transcription factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurofibromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg-Shah and Waardenburg-Hirschsprung disease. Anti-SOX-10 has been shown to be sensitive for conventional, spindled, and desmoplastic melanoma, and has been used to detect metastatic melanoma and nodal capsular nevus in sentinel lymph nodes.
Sulfo DBCO-PEG4-Maleimide is a water-soluble click chemistry reagent with a maleimide group and a DBCO moiety. The hydrophilic PEG spacer arm improves solubility in aqueous buffers. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG4-Maleimide is a water-soluble click chemistry reagent with a maleimide group and a DBCO moiety. The hydrophilic PEG spacer arm improves solubility in aqueous buffers. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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