
Description
Specifications
Clone | IHC626 |
Source | Mouse Monoclonal |
Positive Control | Stomach |
Dilution Range | 1:200 |
Mucin 6 (MUC6) is a glycoprotein expressed in mucous neck cells, pyloric glands of the antrum, epigastric and bronchial epithelium, and in Müller ducts of the endocervix and urethral epithelium. Anti-MUC6 is useful for differentiating fetal, precancerous, and cancerous colonic mucosa from normal colon, as the antibody does not stain the latter. Anti-MUC6 stains the gastric epithelial surface of normal human gastrointestinal tract.
Clone | IHC626 |
Source | Mouse Monoclonal |
Positive Control | Stomach |
Dilution Range | 1:200 |
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
NGS DNA Fragmentation & Library Prep Kit Workflow
The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.
Three index types are available for the kit of the illumina platform:
Non-index (Cat.# 30026): Libraries do not have index.
Index (Cat.# 30028): Each of the index primers contains a unique index sequence of 6 bases. Library multiplexing for 48 samples is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30030): Library multiplexing for 96 samples is possible. With the unique features of our 4-Base Difference Index System, the index sequence is 8-base long and each index has 4 bases different from others. Our unique dual index primers effectively identify sequencing errors such as index hopping, mis-assignment of reads, and de-multiplexing errors etc. The unique dual index primers set consists of 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34028).
Kit features:
Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.
The library size is inversely correlated with the incubation time of step 1 at 20°C.
NGS data comparison: enzymatic shearing versus mechanical shearing
Enzymatic shearing
• DNA shearing and library prep: BioDynami NGS DNA Fragmentation & Library Prep Kit
Mechanical shearing
• DNA shearing: Covaris sonication
• Library prep: BioDynami NGS DNA Library Prep Kit.
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
Cell Washer/Serofuge Centrifuge
Features
1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
3. Special program, HLA and SERO separately.
5. Micro computer control system, LED display the RCF,time and speed.
6.. Electric lid lock, compact design, super speed and imbalance protection.
TD4 Technical Parameter:
Max. Speed | 4000rpm |
Max. RCF | 2000 |
Max. Capacity | 12×7 |
Time Range | 0~99min |
RPM/RCF Convert | Yes |
Noise (dB) | ≤ 55 |
Temperature | Normal |
Acc/Dcc | 10 Kinds |
Speed Accuracy | ±20r/min |
Temperature Accuracy | / |
Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
Size (W x D x Hmm) | 485×320×255mm |
Net Weight(Kg) | 24KG |
Matched Rotors for TD4
Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF(×g) |
NO31501 | SERO Rotor | 3000 | 12×7/5ml(10-13*60-100mm) | 2000 |
NO31502 | HLA Rotor | 4000 | 12×2/1.5/0.5ml | 1000 |
Rotor Name | Max RCF(×g) | Use |
HLA rotor for Lymphocyte washing | 2000 | Lymphocyte separation incubated cell separation |
1000 | Hematoblast extenterate(thrombin disposal) | |
1000 | Lymphocyte washing | |
SERO rotor for erythrocyte washing | 500 | Blood type determination, the image of the hematocyte |
1000 | The test of intersectant aptness | |
1000 | Hematocyte washing, the distillation of the serum and plasma |
TD4 cell washer centrifuge it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.
Standard LyoBeads contain DNA polymerase(s), reaction buffer and dNTPs. LyoBeads can also contain Primer & Probes when produced customized. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very simple. Only a biological sample has to be added.
Storage: LyoBeads are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.
LyoBeads can be pre-dispensed in PCR-strips, PCR-plates, cartridges etc.
All necessary components for PCR are already included in one LyoBead: an engineered DNA polymerase, an optimized reaction buffer and ultrapure dNTPs. Only primers and probes need to be added. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction set-up.
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.
Standard LyoBeads contain DNA polymerase(s), reaction buffer and dNTPs. LyoBeads can also contain Primer & Probes when produced customized. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very simple. Only a biological sample has to be added.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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