Magnetic Beads (PCR Purification)

Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for DNA purification because they are fast, simple and efficient. Our Magnetic Beads (PCR Purification) combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of magnetic beads. The beads are developed for effective PCR fragment purification by removing primers and unwanted components such as salts, dNTPs, enzymes, and others.

Our Magnetic beads (PCR Purification) are optimized to selectively bind PCR fragments of 80 bp and larger and remove primers that are 30 nt and shorter. Purified PCR fragments are suitable for any downstream applications. The beads can be used for PCR cloning, PCR cleanup, or even PCR fragment concentration.

Features:

• Effective purification of PCR fragments >80 bp
• Removal of primers <30 nt
• Removal of unwanted components
• Flexibility: compatible with manual and automated processing

Applications:

• Purification of PCR fragments
• PCR cloning
• Sequencing
• Other applications requiring purified PCR fragments

Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for DNA purification because they are fast, simple and efficient. Our Magnetic Beads (PCR Purification) combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of magnetic beads. The beads are developed for effective PCR fragment purification by removing primers and unwanted components such as salts, dNTPs, enzymes, and others.

Overview

• Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
• No hazardous chemicals required (e.g. phenol or chloroform)
• Effective lysis with Proteinase K and optional lysozyme treatment
• Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
• Wide compatibility with a variety of food products for GMO-DNA isolation
• Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)

This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.

This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping.  

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice)	200 mg 1 mL to 1.5 mL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Samples Tested by PCR

Food Materials	Samples that Have Been Tested by PCR
Plant related	Cereal, Jam, Chocolate, Spices, Sauce
Animal related	Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage)
Dairy product	Milk, Yogurt, Cheese
Pathogens (enriched from food samples)	E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk

Component	Cat. 54500 (50 preps)
Lysis Buffer L	60 mL
Binding Buffer I	7 mL
Buffer SK	30 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Proteinase K	1 vial
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR 

Magnetic beads are pulled midway up onto well wall to help eliminate accidental bead pellet disruption and to achieve lower volumes

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, compatible with any magnetic beads, and integrated cushion base to help aid in robot/ consumable inconsistencies

SKU #

MSP384LE

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• High throughput applications 
• Magnets sit lower on wells allowing lower volumes
• Made in USA 
• For Research use only

Compatibility

• Full- skirt 384 well PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 39 µL 

Minimum  – 5 µL

The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR