GeneAb™ p53

DBCO-PEG2-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG2-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• For use from preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200)
• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Fits into in vitro diagnostic workflows
• Isolate high-quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples
• Fast and easy processing using rapid spin column format
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions
• Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA
• Buffer chemistry inactivates viruses including SARS-CoV-2 – Explore the Application Note
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The chemistry employed in the kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitro diagnostic use for medical purposes.

Saliva/Swab RNA Purification Kit Dx (Spin Column)

This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Saliva/Swab RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s Saliva/Swab RNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Saliva/Swab RNA Purification 96-Well Kit Dx (High Throughput)

This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

• High throughput 96-well plate format for rapid purifications
• Very consistent well-to-well RNA isolation
• Bind & elute all RNA irrespective of size or GC content, without bias

Details

Supporting Data

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Kit Specifications
Sample Volume Range	250 µL
Size of RNA purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	75 µL
Maximum Elution Volume	100 µL
Time to complete 96 purifications	30 minutes
Average Yield	≥ 1 µg **varies from sample to sample

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  All solutions and plastics can be used until the expiration date specified on their labels. 

Component	Cat. Dx69100 (50 preps)	Cat. Dx69300 (192 preps)
Lysis Buffer A	30 mL	100 mL
Solution WN	18 mL	55 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Solution A	6 mL	20 mL
Mini Spin Columns	50	–
96-Well Isolation Plate (Deep Well)	–	2
Collection Tubes	50	–
96-Well Collection Plate (Deep Well)	–	2
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate (Deep Well)	–	2
Adhesive Tape	–	4
Product Insert	1	1

Introduction

Usages:

For the rapid detection and enumeration of coliform bacteria.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the color groups  produce green colonies on the light yellow plate.

Formulation (per liter):

Peptone :10g

Yeast extract powder: 3g

sodium chloride:5g

sodium lauryl sulfate:0.1g

Agar :12g

Chromogenic substrate 2.7g

Final pH 7.0 ± 0.2

How to use:

1. Weigh 32.8g of this product, adding 1L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving.

2,Take 25g or 25mL of sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate buffered saline (or saline) is shaken thoroughly homogenized with a homogenizer or a 1:10 dilution 1min, then 1:10 dilution continue to select the appropriate serial dilutions of three, two plates each dilution was inoculated.

3, the use of pour method: The medium is cooled in a water bath to about 50 , sterilized petri dish having a diameter 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, upside down, 37 for 24 hours.

4, using surface inoculation: cooled to about 50 , shake devoted to the medium in sterile petri dish. Can be stored in the refrigerator for a day or stored at room temperature for several days (dark, 4 ). The sample was streaked method or filter method, 37 for 24 hours.

5, observe the results.

Quality control:

This product appear light yellow after pouring into plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.

Growth of bacteria were cultured bacteria numbers feature

Escherichia coli ATCC25922 good green colonies

Citrobacter ATCC8090 good green colonies

Salmonella typhimurium CMCC50115 good colorless colonies

Enterococcus faecalis ATCC29212 suppressed —–

Storage: Store at 10-30 , dark, cool and dry place, tighten the cap immediately after use. Storage period of two years.

1000mL