Description
Specifications
| Clone | IHC008 |
| Source | Mouse Monoclonal |
| Positive Control | Ovarian Carcinoma (Non-Mucinous Carcinoma), Thyroid Carcinoma, Renal Cell Carcinoma |
| Dilution Range | 1:200 |
PAX-8 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. This protein plays a role in development of thyroid follicular cells and the expression of thyroid-specific genes, with mutations in the PAX-8 gene linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. The PAX-8 protein is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. PAX-8 is also not expressed in normal lung or lung carcinomas. Reports have associated PAX-8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX-8 as a useful marker for renal epithelial tumors, ovarian cancer, and for differential diagnoses in lung and neck tumors. Anti-PAX-8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.
| Clone | IHC008 |
| Source | Mouse Monoclonal |
| Positive Control | Ovarian Carcinoma (Non-Mucinous Carcinoma), Thyroid Carcinoma, Renal Cell Carcinoma |
| Dilution Range | 1:200 |
Product Description
Nucleic Acid Reagent For DNA Extraction: Fast, Efficient, High-Quality
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Features:
Technical Parameters:
| Product Name | Technical Parameters |
|---|---|
| Nucleic Acid Extraction Reagent | Application: DNA Extract Form: Liquid Shelf Life: 12 Months Brand: Amp-future Bio Automated Nucleic Acid Extractor, DNA Extraction Kit, Automated Nucleic Acid Extractor |
Applications:
Amp-future Bio Nucleic Acid Extraction Reagent is a liquid reagent specially formulated for automated nucleic acid extraction. It is widely used in DNA/RNA extraction from various sample matrixes, such as blood, tissue, cells, bacteria, etc. It ensures high-quality nucleic acid extraction in a short period of time. The reagent is produced in China and has a shelf life of 12 months.
Amp-future Bio Nucleic Acid Extraction Reagent is suitable for use with the Automated Nucleic Acid Extractor. It is highly efficient and reliable, allowing for fast and accurate nucleic acid extraction. The reagent is also suitable for use with the DNA Extraction Kit, which makes it ideal for clinical laboratories. It provides superior nucleic acid extraction performance.
Amp-future Bio Nucleic Acid Extraction Reagent is an essential product for laboratories that need to extract nucleic acids from various sample matrixes. It is highly reliable and efficient, providing superior results in a short period of time. The reagent is also suitable for use with the Automated Nucleic Acid Extractor and the DNA Extraction Kit. It is a reliable and cost-effective product for laboratories that require high quality nucleic acid extraction.
Customization:
Customized Nucleic Acid Reagent Service
Amp-future bio provides custom nucleic acid reagent services to meet your specific needs. We specialize in the production of DNA/RNA extraction reagents for automated nucleic acid extraction, purification and other related applications.
Our products are suitable for DNA extraction, automated nucleic acid extraction, nucleic acid purification, and other related applications. We are confident that our products can meet the highest quality standards and provide the best performance. We are dedicated to providing the best customer service and technical support for our customers.
Support and Services:
Our team of experts provide the highest quality technical support and services for Nucleic Acid Reagents products.
Our technical support staff are available to assist you with any questions or troubleshooting related to the use of Nucleic Acid Reagents. Our team can provide guidance on the proper use and handling of our products, as well as any other technical questions. We offer technical support via phone, email, and online chat.
We also offer a variety of services to support our customers in the use of Nucleic Acid Reagents. These services include:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(Nucleic Acid). The HiPure Circulating Nucleic acid Micro Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be either fresh or frozen (provided that they have not been frozen and thawed more than once). Free-circulating cell-free DNA, RNA or viral nucleic acids are eluted in Nuclease Free Water, ready for use in amplification reactions or storage at -30 to -15°C. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 0.6ml plasma,serum, body fluids |
| Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Serum, plasma and other cell-free fluid samples |
| Sample amount | 0.6ml |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based onsilica column purification. The sample is lysed and digested with lysate andprotease, DNA is released into the lysate. Transfer to an adsorption plate andfilter column. Nucleic acid is adsorbed on the membrane, while protein is notadsorbed and is removed with filtration. After washing proteins and otherimpurities, Nucleic acid was finally eluted with low-salt buffer.
Kit Contents
| Contents | D318002 | D318003 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer ACL | 40 ml | 200 ml |
| Buffer DCW1 | 22 ml | 110 ml |
| Buffer DCW2* | 20 ml | 2 x 50 ml |
| Proteinase K | 34 mg | 180 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Carrier RNA | 110 μg | 310 μg |
| Nuclease Free Water | 10 ml | 30 ml |
| HiPure CFDNA Mini Columns | 50 | 250 |
| 2 ml Collection Tubes | 100 | 5 x 100 |
Storage and Stability
Proteinase K, Carrier RNAshould be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15-25°C) andare stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use.Make sure that all buffers are at room temperature when used.
Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
TDL5B refrigerated low speed centrifuge
Technical Parameters
| Max speed： | 5000rpm | Max RCF： | 4730xg |
| Max volume | 4x800ml | Noise： | ≤65dBA |
| Timer | 1～99h59min | Net weight： | 125 Kg |
| Dimension(HxDxW)： | 430×688×715mm | Power supply | AC 220V 50HZ 15A |
| Temperature range | -20℃~40℃ | Temperature accuracy | ±1℃ |
| Speed accuracy： | ±20rpm | Package | wooden box |
Matched Rotors for TDL5B
| Order No. | Model | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| No.30671 | Swing out rotor | 4000 | 4x800ml (round cup) | 3450 |
| Adapters for 4x800ml (round cup) swing rotor for 5ml, 7ml, 10ml, 15ml, 50ml, 100ml, 250ml, 500ml tubes | ||||
| No.30696 | Swing out rotor | 4000 | 4x500ml (square cup) | 3310 |
| No.31277 | Swing out rotor body | |||
| Matched Buckets for the swing out rotor body | 5000 | 4x50ml | 4420 | |
| 4x100ml | ||||
| 4000 | 8x50ml | 2950 | ||
| 16x15ml | ||||
| 24x15ml | ||||
| 4000 | 24×10/7ml vacuum tube | 2580 | ||
| 16x10ml vacuum tube | ||||
| Order No. | Model | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| No.31377 | Matched Buckets for the swing out rotor body | 5000 | 32x10ml vacuum tube | 2580 |
| 32x7ml vacuum tube adapter | ||||
| 32x5ml vacuum tube adapter | ||||
| 24x10ml vacuum tube | 2580 | |||
| 24x7ml vacuum tube adapter | ||||
| 24x5ml vacuum tube adapter | ||||
| 16x10ml vacuum tube | 2580 | |||
| 16x7ml vacuum tube adapter | ||||
| 16x5ml vacuum tube ada |