Description
Specifications
| Clone | IHC654 |
| Source | Mouse Monoclonal |
| Positive Control | Prostate, Prostate Carcinoma |
| Dilution Range | 1:200 |
Prostate-Specific Antigen (PSA) is a serine protease of the kallikrein family, that is produced by the prostate epithelium and epithelial lining of the periurethral glands. Although considered prostate-specific, PSA has also been detected in breast tissue, breast tumors, endometrium, adrenal neoplasms, and renal cell carcinomas. Anti-PSA can be used for differentiating high-grade prostate adenocarcinoma from high-grade urothelial carcinoma, as well as for determining the prostatic origin of carcinomas in non-prostate tissues. Anti-PSA recognizes primary and metastatic prostatic neoplasms, but not tumors of nonprostatic origin, and can be useful as an aid to confirm prostatic acinar cell origin in primary and metastatic carcinomas.
| Clone | IHC654 |
| Source | Mouse Monoclonal |
| Positive Control | Prostate, Prostate Carcinoma |
| Dilution Range | 1:200 |
Usages:
For differentiation of enterobacteriaceae.
Principle:
Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
Formulation(per liter):
Peptone 20g
Beef extract powder 3g
Yeast extract powder 3g
Lactose 10g
Dextrose 1g
Sodium chloride 5g
Ferric ammonium citrate 0.5g
Sodium thiosulfate 0.5g
Agar 12g
Phenol red 0.05g
Final pH 7.4 ± 0.2
How to use:
1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Quality control:
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Escherichia coli ATCC25922 | Good | A/A |
| 2 | Proteus CMCC (B) 49027 | Good | K/A |
| 3 | Salmonella typhimurium CMCC (B) 50115 | Good | K/A |
“A”Acid , “K” Alkality
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
This product supplies a simple and rapid extraction of total RNA including microRNA from Blood, buffy coat, bone marrow, Cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on.
The Kit can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤ 1 x106 cells suspension, 50 μl Whole Blood, 50μl buffy coat, 20μl bone marrow. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and
Kit Contents
| Contents | R661101C | R661102C | R661103C |
| Purification times | 48 Preps | 96 Preps | 480 preps |
| MagPure RNA Particles | 1.0 ml | 1.5 ml | 6 ml |
| Proteinase K | 24 mg | 48 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 15 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 µl |
| DNase Buffer | 15 ml | 15 ml | 60 ml |
| Buffer AL | 10 ml | 10 ml | 60 ml |
| Buffer MCB* | 9 ml | 9 ml | 30 ml |
| Buffer MW1* | 13 ml | 22 ml | 110 ml |
| Buffer MW2* | 6 ml | 20 ml | 100 ml |
| RNase Free Water | 10 ml | 15 ml | 120 ml |
Storage and Stability
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.
This product supplies a simple and rapid extraction of total RNA including microRNA from Blood, buffy coat, bone marrow, Cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on.
The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR
Magnetic beads are pulled midway up onto well wall to help eliminate accidental bead pellet disruption and to achieve lower volumes
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, compatible with any magnetic beads, and integrated cushion base to help aid in robot/ consumable inconsistencies
MSP384LE
Maximum – 39 µL
Minimum – 5 µL
The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR