
Description
Specifications
Clone | IHC655 |
Source | Mouse Monoclonal |
Positive Control | Prostate, Prostate Adenocarcinoma |
Dilution Range | 1:200 |
Prostatic Specific Acid Phosphatase (PSAP) is a prostatic enzyme found in the glandular epithelium of the prostate. PSAP levels are elevated in hyperplastic prostate and prostate carcinoma, with the highest levels being detected in metastasized prostate cancer. Moderate overexpression of PSAP is also characteristic of diseases of the bone (such as Paget’s disease or hyperparathyroidism), diseases of blood cells (such as sickle-cell disease), multiple myeloma, or lysosomal storage diseases (such as Gaucher’s disease). PSAP is considered more sensitive, yet less specific, than PSA, however Anti-PSAP can act as a useful complement to Anti-PSA under suitable clinical contexts.
Clone | IHC655 |
Source | Mouse Monoclonal |
Positive Control | Prostate, Prostate Adenocarcinoma |
Dilution Range | 1:200 |
This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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Kit Specifications – Spin Column | |
Column Binding Capacity | 13 μg |
Maximum Column Loading Volume | 650 μL |
Maximum Amount of Starting Material | 25 μg |
Time to Complete 10 Purifications | 10 minutes |
Minimum Elution Volume | 30 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 34500 (50 preps) | Cat. 34400 (2 x 96-well plate) |
---|---|---|
Binding Buffer C | 30 mL | 3 x 30 mL |
Wash Solution A | 12 mL | 2 x 20 mL |
Elution Buffer B | 8 mL | 2 x 15 mL |
Spin Columns | 50 | – |
Collection Tubes | 50 | – |
Elution Tubes (1.7 mL) | 50 | – |
96-Well Plate | – | 2 |
Adhesive Tape | – | 4 |
96-Well Collection Plate | – | 2 |
96-Well Elution Plate | – | 2 |
Product Insert | 1 | 1 |
DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
---|---|---|
DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
Buffer DR | 1 mL | 4 x 1 mL |
Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
Product Insert | 1 | 1 |
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Email : hej@a3p-scientific.com
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