
Description
Specifications
Clone | IHC655 |
Source | Mouse Monoclonal |
Positive Control | Prostate, Prostate Adenocarcinoma |
Dilution Range | 1:200 |
Prostatic Specific Acid Phosphatase (PSAP) is a prostatic enzyme found in the glandular epithelium of the prostate. PSAP levels are elevated in hyperplastic prostate and prostate carcinoma, with the highest levels being detected in metastasized prostate cancer. Moderate overexpression of PSAP is also characteristic of diseases of the bone (such as Paget’s disease or hyperparathyroidism), diseases of blood cells (such as sickle-cell disease), multiple myeloma, or lysosomal storage diseases (such as Gaucher’s disease). PSAP is considered more sensitive, yet less specific, than PSA, however Anti-PSAP can act as a useful complement to Anti-PSA under suitable clinical contexts.
Clone | IHC655 |
Source | Mouse Monoclonal |
Positive Control | Prostate, Prostate Adenocarcinoma |
Dilution Range | 1:200 |
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
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Kit Specifications | |
---|---|
Sample Type | Fresh or frozen urine |
Sample Volume Range | 250 μL to 2mL |
Minimum Elution Volume | 50 μL |
Maximum Elution Volume | 100 μL |
Time to Complete 10 Purifications | 25–30 minutes |
Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Average Yields ¥ | Variable depending on specimen |
¥Please check page 5 of protocol for average urine yields and common RNA quantification methods.
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Mini (Cat. 72900) | Midi (Cat. 72910) | Maxi (Cat. 72920) |
---|---|---|---|
Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
Lysis Buffer A | 30 mL | 20 mL | 20 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Collection Tubes | 50 | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
EXTRAClean Mini Spin Columns | 50 | 20 | 10 |
EXTRAClean Midi Spin Columns | – | 20 | – |
EXTRAClean Maxi Spin Columns | – | – | 10 |
Product Insert | 1 | 1 | 1 |
Usages:
Pancreatic digest of casein obtained with a process to maintain a high level of vitamins and growth factors, it is particularly recommended for a rapid growth and detection of microorganisms present in low concentrations.
Technical specification:
Total nitrogen(TN)—————————≥11.5%
Amino nitrogen(AN) ————————≥3.0%
Sulphuri ash———————————–≤15.0%
Loss on drying——————————–≤6.0%
Chlorides—————————————≤2.0%
PH(5% solution)——————————7.0±0.5
Solubility in 5% water ———————–complete
Nitrites——————————————negative
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: 500g/bottle; 10kg/bag
500g/bottle;10KG/bag
PEG3-bis(Amino-Tri-(Propargyl-PEG8-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a short PEG linker. The alkynes are most frequently used in copper click chemistry with azides to form stable triazoles with the target compound. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter DMPK of this compound.
PEG3-bis(Amino-Tri-(Propargyl-PEG8-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a short PEG linker. The alkynes are most frequently used in copper click chemistry with azides to form stable triazoles with the target compound. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter DMPK of this compound.
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