Description
Specifications
| Clone | IHC072 |
| Source | Mouse Monoclonal |
| Positive Control | Lung Adenocarcinoma |
| Dilution Range | 1:200 |
Tumor-Associated Glycoprotein 72 (TAG-72) is a glycoprotein found on the surface of many cancer pathologies. Anti-TAG-72 can be useful for detecting some adenocarcinomas and non-neoplastic tissues. This diagnostic grade TAG-72 IVD antibody is useful for identifying adenocarcinomas in positive staining, but in mesotheliomas no staining is observed.
| Clone | IHC072 |
| Source | Mouse Monoclonal |
| Positive Control | Lung Adenocarcinoma |
| Dilution Range | 1:200 |
Chlamydia trachomatis, the causative agent of Chlamydia, is a Gram negative bacteria. Transmission of the bacteria occurs via contact with infected bodily fluids which then infect mucosal membranes. It can be transmitted from mother to child during pregnancy and infect the eyes causing conjunctivitis. The genital infection causes urethritis, epididymitis and prostatitis in males and Pelvic Inflammatory Disease (PID) in females with an increased risk of contracting HIV. The infection can be treated with a course of antibiotics. Sexually transmitted infections in females are most often asymptomatic, but can be noticeable in chronic pain of the pelvic region, vaginal bleeding and painful urination. Infection of the ovaries, fallopian tubes or uterus causes Pelvic Inflammatory Disease (PID) which can lead to difficulties in conceiving, increased risk of ectopic pregnancy or infertility. Infections in males are more likely to be symptomatic, causing painful urination, discharge from the penis and swollen testicles and may eventually cause infertility if left untreated.
Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is transmitted by sexual contact and usually causes infection in cells of the mucous membrane of the male urethra or the endocervix and urethra in females. During infection, polysaccharides are released from the bacteria that stimulate host cell production of tumour necrosis factors that cause an inflammatory response. There is no vaccine against N. gonorrhoeae infection and antibiotic resistance is beginning to increase, therefore treatment includes a course of antibiotics that will be effective against resistant strains. Complications in males caused by the infection can result in prostatitis or orchitis if the bacteria spread. In females, invasion of the fallopian tubes or ovaries can result in salpingitis or ovaritis respectively, with any of these infections possibly resulting in sterility.
Chlamydia/Neisseria TaqMan PCR Kit,
100 reactions
Chlamydia/Neisseria TaqMan PCR Probe/Primer Set and Controls,
100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM42550 (100 preps) | Cat. TM42510 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Chlamydia/Neisseria Primer & Probe Mix | 280 μL | 280 μL |
| Chlamydia/Neisseria Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Propargyl-PEG5-Ms represents a bifunctional linker possessing a proparygyl group reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound as well as a mesyl group which is a good leaving group for nucleophilic reactions.
Propargyl-PEG5-Ms represents a bifunctional linker possessing a proparygyl group reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound as well as a mesyl group which is a good leaving group for nucleophilic reactions.
Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.
Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.
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| Kit Specifications | |
| Resin Binding Capacity (total per kit) | At least 3×1011 adenovirus particles as determined by qPCR At least 3×108 transducing units as determined by transduction assay |
| Input type | Cells, media |
| Input volume (Supernatant) | 1 – 33.5 mL SN per prep (500 mL SN in total) |
| Input Volume (Cell pellet) | 1 mL cell pellet per prep (15 mL in total) |
| Minimum elution volume | 1.3 mL per prep |
| Time to complete purification | 2.5 to 4.5 hours with 1 hour hands on time |
| In vivo transduction | Yes |
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 67600 (15 preps) |
|---|---|
| Lysis Buffer S | 5.5 mL |
| HL-SAN Nuclease | 102 µL |
| Binding Buffer A | 20 mL |
| Purification Solution C | 60 mL |
| Purification Solution D | 130 mL |
| Wash Solution C | 2 x 130 mL |
| Slurry E | 12.5 mL |
| Elution Buffer P (store at 4°C) | 66 mL |
| Protein Neutralizer | 4 mL |
| Elution tubes (1.7 mL) | 50 |
| Product Insert | 1 |
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