
Description
Specifications
Clone | IHC072 |
Source | Mouse Monoclonal |
Positive Control | Lung Adenocarcinoma |
Dilution Range | 1:200 |
Tumor-Associated Glycoprotein 72 (TAG-72) is a glycoprotein found on the surface of many cancer pathologies. Anti-TAG-72 can be useful for detecting some adenocarcinomas and non-neoplastic tissues. This diagnostic grade TAG-72 IVD antibody is useful for identifying adenocarcinomas in positive staining, but in mesotheliomas no staining is observed.
Clone | IHC072 |
Source | Mouse Monoclonal |
Positive Control | Lung Adenocarcinoma |
Dilution Range | 1:200 |
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from FFPE tissue samples |
Applications | PCR, Southern Blot and viral DNA detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples |
Sample amount | <20mg |
Elution volume | >15µl |
Time per run | ≤20 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 100μg |
Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After decrosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.
Contents | IVD3126 |
Purification Times | 100 Preps |
HiPure DNA Mini Columns I | 100 |
2ml Collection Tubes | 100 |
Buffer DPS | 70 ml |
Buffer ATL | 30 ml |
Buffer AL | 30 ml |
Buffer GW1* | 44 ml |
Buffer GW2* | 20 ml |
Proteinase K | 50 mg |
Protease Dissolve Buffer | 6 ml |
Buffer AE | 20 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Norgen’s Saliva RNA Collection and Preservation Devices are designed for simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices consists of 3 components:
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.
Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature.
Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300).
Figure 1 / 4
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Kit Specifications | |
Volume of Saliva Collected | 2 mL |
Volume of Saliva-Preservative Mix | 4 mL |
Preservation Temperature | Room Temperature |
Preservation time | Up to 2 months at room temperature |
Shelf Life and Handling
Kit Components | Cat. RU53800 (50 Devices) |
---|---|
Individual Saliva RNA Collection and Preservation Devices | 50 |
Donor Procedure Flowchart | 1 |
Product Insert | 1 |
Individual Saliva RNA Collection and Preservation Device Contents | |
Saliva Collection Funnel and Collection Tube | 1 |
Collection Tube Cap | 1 |
Preservative Ampoule | 1 |
Donor Instructions | 1 |
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