GeneAb™ TAG-72

Description

The Benzoic Acid Detection Kit provides a rapid, simple, sensitive, and reliable test suitable for screening of Benzoic Acid concentration.

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Benzoic Acid is a white solid that is an extensively used preservative.  Although this preservative prevents or delays nutritional losses due to microbiological, enzymatic or chemical changes of foods during its shelf life there is a suspicion that small amounts of benzene may be formed from benzoic acid in nonalcoholic beverages in the presence of ascorbic acid. Benzoic acid and ascorbic acid are food additives which must be declared on the food.  Benzoic acid or E 210 is a preservative which also occurs naturally, for instance, in cranberries. A maximum amount of 150 mg/l benzoic acid may be added to non-alcoholic flavored beverages.

Highly Sensitive Assay to Screen for Benzoic Acid
Visual Readout (sample dependent: milk, red/pink)
Detection range of 1ppm to 1500ppm
Tube or Plate based options available
Compatible with the Nix Sensor or plate readers to obtain quantitative results.

Overview

• Detection kits for Streptococcus uberis
• Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.

Streptococcus uberis TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Streptococcus uberis TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM30850 (100 preps)	Cat. TM30810 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
S. uberis Primer & Probe Mix	280 μL	280 μL
S. uberis Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request