Description
Specifications
| Clone | IHC969 |
| Source | Mouse Monoclonal |
| Positive Control | Thyroid Gland |
| Dilution Range | 1:200 |
Tau proteins are abundant in neurons of the central nervous system, and function in microtubule stability and organization. Defects in Tau functioning and aggregation of these proteins have been clinically linked to a number of neurodegenerative disorders including Alzheimer’s disease, Parkinson’s disease, Pick’s disease (PiD), progressive supranuclear palsy (PSP), cortical basal degeneration (CBD), and frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17).
| Clone | IHC969 |
| Source | Mouse Monoclonal |
| Positive Control | Thyroid Gland |
| Dilution Range | 1:200 |
Peelable heat sealing foil which seals to polystyrene plates. This seal is resealable, pierceable and suitable for compound storage too.
These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)
This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.
Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)
Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.
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| Kit Specifications | |
| Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material: Fungi (Wet weight) Yeast or Gram-positive bacterial culture | 50 mg 0.5 mL – 1 mL |
| Time to Complete 96 Purifications | 40 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 27300 (50 preps) | Cat. 27350 (192 preps) |
|---|---|---|
| Lysis Buffer L | 30 mL | 2 x 60 mL |
| Resuspension Solution A | 20 mL | 60 mL |
| Solution BX | 28 mL | 2 x 28 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Buffer B | 15 mL | 30 mL |
| Bead Tubes | 50 | 200 |
| Spin Columns | 50 | – |
| Collection Tubes | 50 | – |
| 96-Well Plate | – | 2 |
| 96-Well Collection Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Elution Plate | – | 2 |
| Product Insert | 1 | 1 |
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from FFPE tissue samples |
| Applications | PCR, southern blot and viral DNA detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples |
| Sample amount | <20mg |
| Elution volume | >15µl |
| Time per run | ≤20 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 100μg |
Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.
| Contents | D312602 | D312603 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer DPS | 30 ml | 150 ml |
| Buffer ATL | 15 ml | 60 ml |
| Buffer AL | 15 ml | 60 ml |
| Buffer GW1* | 22 ml | 88 ml |
| Buffer GW2* | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 10 ml | 30 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.