Isolate genomic DNA from animal tissues, cells, bodily fluids, viruses and swabs
Rapid and convenient spin column procedure
Purified DNA is of the highest quality and integrity for sensitive downstream applications including PCR, qPCR, genotyping, sequencing and more
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney)
8 µg 10 µg
Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension
20 mg 3 x 106 cells 150 µL 150 µL
Time to Complete 10 Purifications
80 minutes
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component
Cat. 24700 (50 preps)
Cat. 24750 (100 preps)
Cat. 24770 (250 preps)
Digestion Buffer A
25 mL
2 x 25 mL
5 x 25 mL
Buffer SK
30 mL
2 x 30 mL
5 x 30 mL
Wash Solution A
18 mL
2 x 18 mL
5 x 18 mL
Elution Buffer B
30 mL
2 x 30 mL
5 x 30 mL
Proteinase K
12 mg
2 x 12 mg
5 x 12 mg
Spin Columns
50
100
250
Collection Tubes
50
100
250
Elution Tubes (1.7 mL)
50
100
250
Product Insert
1
1
1
Other Products
Propargyl-PEG5-CH2CO2tBu
Product Info
Document
Product Info
Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
Isolate total RNA, including microRNA, without phenol
Rapid and convenient spin-column format and 96-well plate for high throughput applications
Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
*Additional RBC Lysis Solution is required for input Volumes >150 µL
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
The Hepatitis B virus (HBV) is mainly transmitted via blood or blood products. In addition, sexual, oral and perinatal infections are also possible. Early symptoms of the infection include appetite loss, vomiting and abdominal symptoms, with approximately 10-20% of those patients developing fever as well as rheumatoid joint and muscle pain. Jaundice, which may be accompanied by itching, will then develop within 2-14 days. Fulminant hepatitis then occurs in about 1% of all infected patients, which in severe cases may be fatal. Of those individuals infected by HBV, 5-10% will develop chronic liver inflammation which may progress to cirrhosis of the liver or, in the worst case, primary liver cell carcinoma. The HBV virus is a hepadnavirus that has a circular genome composed of partially double-stranded DNA.
HBV TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
