Glucomannan Assay Kit

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

Detail

K-GLUM

SKU: 700004300

50 Assays per kit

Content:	50 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Glucomannan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 μg of glucomannan per assay
Limit of Detection:	1 g/100 g
Total Assay Time:	120 min
Application examples:	Jelly sweets, cosmetics, food gums and other materials.
Method recognition:	*Novel method*

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

View more of our polysaccharide test kits.

Scheme-K-GLUM GLUM Megazyme

Advantages

Very cost effective
Only enzymatic kit available
Simple format
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

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Extract high quality & quantity total RNA including miRNA
No phenol step required; isolate all RNA in one fraction
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Isolate from a wide variety of specimens
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Available in a variety of formats to suit your needs
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A₂₆₀/A₂₈₀ suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Details

Supporting Data

Figure 1 / 13

Figure 1. High Quality of Isolated RNA with Complete Size Range.

Figure 1. High Quality of Isolated RNA with Complete Size Range.

Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction.

Figure 3. High Quality of RNA from a Diverse Range of Inputs.

Figure 4. Great Isolation Sensitivity.

Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA.

Figure 6. Effective and Consistent Detection of miRNA from Plasma.

Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency.

Figure 8. Better Diversity of miRNA Detected from Plasma.

Figure 9. Better Diversity of miRNA Detected from HeLa Cells.

Figure 10. Total RNA Purification 96-Well Kit

Figure 11. Total RNA Purification 96-Well Kit

Figure 12. Total RNA Purification 96-Well Kit

Figure 13. Total RNA Purification 96-Well Kit

Click for expanded view

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Amount of Starting Material
Animal Cells	1 x 10⁶ cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 10⁶ cells)	15μg
E. coli Cells (1 x 10⁹ cells)	50 μg

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1

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