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Bacterial DNA Extraction Kit (Magnetic Beads)
Product Info
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Product Info
Bacterial DNA Extraction Kit (Magnetic Beads)
The Bacterial DNA Extraction Kit (Magnetic Beads) was developed for the bacterial genomic DNA extraction from bacterial cultures directly using magnetic beads from a wide variety of gram-negative and gram-positive bacterial species, as well as yeast and other fungi. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality bacterial DNA isolation with fast magnetic response time and high binding capacity.
Bacteria are the most diverse and abundant small single-celled organisms and are vital to the planet’s ecosystems. Some bacterial species are pathogens that can cause a variety of diseases to humans and animals. Besides their ecological and biomedical importance, bacteria are also used in biotech and pharmaceutical applications such as production of enzymes, DNA preparation, biofuels, food research, and chemical production.
Bacterial cells are grown to log-phase and the culture is lysed directly with a lysis buffer, then mixed with beads to bind genomic DNA. After wash steps, genomic DNA is eluted in Low TE or TE Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities. Bacterial DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, Southern Blotting, molecular cloning, DNA hybridization, restriction enzymatic digestion, and Next-generation Sequencing (NGS) etc.
Features
100% centrifuge-free
Bacterial cultures can be used directly without centrifuge to pellet the bacteria
Simple magnetic beads method
No centrifuge needed
No column needed
No vacuum needed
Purified genomic DNA from various bacteria were isolated with the Bacterial DNA Extraction Kit. A portion of the extracted genomic DNA was loaded on a 1% agarose gel. DNA ladder: BioDynami 1 kb Plus DNA Ladder (Cat.# 10005L).
Sequentially isolate and purify total RNA and DNA from a single sample
Two column system: one for DNA and one for RNA
The RNA column is for the purification of total RNA including microRNA
No need to split the lysate, or to use phenol or precipitation methods
Rapid and efficient spin column procedure – it takes only 30 minutes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. The lysate is passed over two columns: 1) a DNA column and 2) an RNA column. Total RNA of all sizes is purified, including microRNA. Both DNA and RNA are of the highest purity and yield.
These kits are ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as they eliminate the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications
RNA/DNA Purification Kit (Spin Column)
Maximum column binding capacity of 50 μg for RNA and 20 μg for DNA.
RNA/DNA Purification Micro Kit (Micro)
The purified RNA and DNA fractions can be eluted in as little as 20 μL. Ideal for cell number inputs of 500,000 and as little as 5 cells. Maximum column binding capacity of 35 μg for RNA and 10 μg for DNA.
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues
5 x 106 cells 25 mg (for most tissues)** 200 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg
Time to Complete 10 Purifications
30 minutes
Average Yield*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg)
10-15 μg RNA 2-4 μg DNA 30-35 μg RNA 4-6 μg DNA
*Average Yield will vary depending upon a number of factors including species, growth conditions used, and development stage.
**Tissue inputs of up to 40 mg may be used, however for inputs greater than the recommended 25 mg, cross-contamination of the RNA and DNA fractions is possible.
Storage Conditions and Product Stability Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 1mg plasmid DNA from 200ml bacterial culture
Applications
Enzyme digestion, sequencing, PCR and labeling, etc.
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid, plasmid less than 30KB
Sample amount
100-200ml
Yield
0.4-1mg
Elution volume
≥500μl
Time per run
≤60 minutes
Liquid carrying volume per column
20ml
Binding yield of column
1mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – it takes only 15-60 minutes to complete the isolation
Economy – high cost performance
Kit Contents
Contents
P101402
P101403
Purification Times
10 Preps
50 Preps
RNase A
20 mg
40 mg
Buffer P1
140 ml
2 x 350 ml
Buffer P2
140 ml
2 x 350 ml
Buffer LEN3
70 ml
350 ml
Buffer GBT
120 ml
550 ml
Buffer PW1
60 ml
300 ml
Buffer PW2*
50 ml
4 x 100 ml
Elution Buffer
20 ml
120 ml
HiPure DNA Maxi Columns III
10
50
Lysate Clear Maxi Syringe
10
50
50 ml Collection Tubes
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.
