
Gly-PEG3-endo-BCN is a click chemistry reagent. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Gly-PEG3-endo-BCN is a click chemistry reagent. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Gly-PEG3-endo-BCN is a click chemistry reagent. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
Applications | PCR, qPCR, southern bolt and virus detection, etc |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples |
Sample amount | Solid tissue: 1-10mg; Anticoagulant blood: 200μl |
Elution volume | ≥20μl |
Time per run | 30 – 60 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Kit Contents
Contents | D301802 | D301803 |
Purification Times | 50 | 250 |
HiPure DNA Mini Columns I | 50 | 250 |
2ml Collection Tubes | 100 | 500 |
Buffer ATL | 30 ml | 150 ml |
Buffer AL | 30 ml | 150 ml |
Buffer GW1 | 22 ml | 88 ml |
Buffer GW2 | 12 ml | 50 ml |
Proteinase K | 24 mg | 120 mg |
Protease Dissolve Buffer | 1.8 ml | 10 ml |
Buffer AE | 15 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Specifications
Features | Specifications |
Concentration | 10 mg/ml |
Appearance | Suspension of yellowish brown particles |
Surface functional group | Carboxyl, COOH |
Dispersibility | Monodisperse, spherical |
Particle size | 0.8-1 μm |
Preservation conditions | Room temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
Magnetic response speed | 120 seconds |
Settling velocity | >2 hours |
High salt mediated binding | No adsorption |
Alcohol mediated binding | 1M NaClO4/ethanol(50%), DNA/RNA recovery up to 90% |
PEG8000 mediated binding | The recovery of DNA/RNA was up to 90% |
DNase/RNase | Not detected |
DNA residue | Not detected |
Recommended application | Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction. |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
CAT.No. | Product Name | Package |
C14130 | MagBind Particles | 10 ml |
C14131 | MagBind Particles | 100 ml |
Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Specifications
Features | Specifications |
Main Functions | Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant. |
Applications | RT-PCR,PCR |
Products | Pathogen DNA / RNA |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant |
Sample amount | 200-300μl |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Kit Contents
Contents | IVD6672-50 | IVD6672 |
Purification Times | 50 Preps | 200 Preps |
Bead Tube C | 50 | 4 x 50 |
MagPure Particle | 1.6 ml | 7.0 ml |
Proteinase K | 24 mg | 100 mg |
Protease Dissolve Buffer | 1.8 ml | 6 ml |
Buffer MLBN | 30 ml | 120 ml |
Buffer MW1* | 22 ml | 53 ml |
Buffer MW2* | 20 ml | 50 ml |
Buffer NFW | 10 ml | 30 ml |
Storage and Stability
MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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