Functionally tested GAM conjugated gold
Perfect for Lateral Flow Development and use in final product
40nm gold particles conjugated to GAM are offered in 10 OD 1mL size. If a different OD or amount is required please
Goat Anti-Rabbit (GAR) Conjugated Colloidal Gold for Lateral Flow (1mL of 10OD)
Product image shows functional testing of Goat Anti Mouse (GAM) 40nm colloidal gold on a lateral flow test.
Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
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P1157 HiPure Plasmid EF 96 Kit
Product Info
Document
Product Info
Introduction
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate
Applications
Cell transfection, animal injection, etc.
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Low copy plasmid vector
Sample amount
1-5ml LB(x96)
Yield
30μg
Elution volume
≥75μl
Time per run
≤60 minutes
Liquid carrying volume per column
800μl
Binding yield of column
70μg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
1. High throughput: processing 96 samples at once; 2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit; 3. Detoxification: endotoxin content <0.1EU/μg; 4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;
Kit Contents
Contents
P115701
P115702
P115703
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
RNase A
10 mg
20 mg
100 mg
Buffer P1
30 ml
120 ml
550 ml
Buffer P2
30 ml
120 ml
550 ml
Buffer LEN3
15 ml
60 ml
300 ml
Buffer LN4
80 ml
270 ml
2 x 700 ml
Buffer LN5
40 ml
220 ml
1100 ml
Buffer PW1
40 ml
220 ml
1100 ml
Buffer PW2
25 ml
100 ml
2 x 200 ml
Elution Buffer
30 ml
60 ml
250 ml
Lysate Clear Plate
1
4
20
HiPure DNA Plate
1
4
20
1.6ml Collection Plate
2
8
40
0.5ml Collection Plate
1
4
20
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Widely used in the area of laboratory, pharmaceutical factory, biochemistry, biological products and so on. It is the ideal instruments for blood separation, protein precipitation and cell collection.
1. Brushless DC motorin great torque, free maintenance, no carbon dust pollution, quick in speed up and down. 2. Flexible axledriven system which drive the rotor directly, smooth in operation, low noise and small vibration. 3. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. 4. Microprocessor control, speed, time, temperature and RCF in operation, speed rising and reducing quick, operate simply. 5.10 kinds of program storedin the memory, 10 kinds of accelerating and decelerating speed for your choice. 6. There are many rotors for your choice, adapters are available by experiment requirements. 7.3 tiers protection steel cover, safe and reliable.
Technical Parameters
for 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine
Max Speed: 6000r/min
Max.RCF: 6600xg
Max Volume: 4x1000ml
Power supply:AC220V,50HZ,10A
Timer: 1~99min
Noise:≤55dBA
Dimension:800×645×830mm
Net Weight: 170KG
Speed Accuracy: ±20r/min
Package(W*D*H): 920×800×1320mm
Temperature range:
Inapplicable
Matched Rotorsfor DD6 laboratory centrifuge
Order No.
Rotor type
Max.Speed (rpm)
Max.Volume (ml)
Max.RCF (xg)
No33097
Swing out rotor
4,000
4x1000ml (round/square cup )
4,060
No30221
Angle rotor
6,000
4x300ml
5,390
No30222
Angle rotor
6,000
6x300ml
5,660
No30223
Angle rotor
6,000
6x500ml
6,600
Packing & Shipping_____________________
by Sea/ by Air / by Express Sevices DHL, Fedex, TNT…
Document
DD6 is floor standing large capacity 4x1000ml, 6x300ml, 6x500ml laboratory centrifuge machine without refrigerated one. It is up to max.speed 6000rpm.
DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety and an azide reactive moiety. DADPS probe can be used in biomolecular labeling and proteomic studies. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety and an azide reactive moiety. DADPS probe can be used in biomolecular labeling and proteomic studies. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.