Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 5ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual (centrifugation or vacuum)
Sample type
Serum, plasma
Sample amount
5ml
Elution volume
≥40μl
Time per run
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
1292750
12927200
Purification Times
50
200
MagPure Particles G
20 ml
80 ml
MagBind Particles (selection particles)
14 ml
58 ml
Selection Solution
100 ml
400 ml
Proteinase K
300 mg
1.2 g
Protease Dissolve Buffer
25 ml
100 ml
Buffer SDS(20%)
15 ml
60 ml
Buffer MLK
300 ml
3 x 450 ml
Buffer BST1
225 ml
2x 450 ml
Buffer MKW1
225 ml
2x 450 ml
Buffer MW2*
50 ml
2x 100 ml
Buffer AE
10 ml
30 ml
Storage and Stability
MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at roomtemperature (15–25°C) and are stable for at least 18 months underthese conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample)
Applications
SDS-PAGE electrophoresis and western blot, etc
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples
Sample amount
Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7
Principle
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R521102
R521103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer GW1*
22 ml
66 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
50 ml
3 x 50 ml
RNase Free Water
10 ml
30 ml
Elution Buffer
10 ml
30 ml
Buffer ALO(5%SDS)
10 ml
30 ml
Storage and Stability
HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Document
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
