Usages: Beef extract powder is a purified extract of beef .Used with meat and casein peptones as a source of nutrients in culture media .
Technical specification: Total nitrogen(TN)—————————≥12.0% Amino nitrogen(AN) ————————≤2.7% Chlorides————————————–<6% Moisture—————————————≤5.0% PH———————————————–6-7
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: 500g/bottle; 10kg /bag
Other Products
IVD3020B MagPure Universal RNA Kit B
Product Info
Document
Product Info
Introduction
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (<10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from tissue, cell, whole blood
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
Tissues, cells, lymphocytes and other clinical sample
Sample amount
Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture
Kit Contents
Contents
IVD3020B-96
IVD3020B
Purification Times
96 Preps
200 Preps
MagPure Particles N
2.5 ml
5 ml
DNase I
2 x 600 µl
4 x 600 µl
DNase Buffer C
60 ml
120 ml
Buffer RLC
60 ml
120 ml
Buffer MCB*
60 ml
150 ml
Buffer GW1*
44 ml
110 ml
Buffer MW2*
50 ml
100 ml
RNase Free Water
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.
Document
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (<10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Cluster of differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid, which plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Studies have suggested that the CD44-hyaluronate interaction is central to tumor invasiveness. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as Non-Hodgkin’s Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumors. It has also been demonstrated that there is a positive correlation between tumor progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma in situ from non-neoplastic changes in the urothelium.
The ITS1 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS1 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal/swab, plasma/serum, tongue swab, gum swab, and others.
The ITS1 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS1 region of the fungal DNA.The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s ITS1 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components will remain stable for at least 1 year when stored at the specified storage conditions.