Usages: For determination of total bacterial count .
Principle: Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent. Formulation(per liter): Tryptone 5.0g Yeast extract powder 2.5g Glucose 1.0g Agar 15.0g Final pH 7.0 ± 0.2
How to use: 1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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PCR Ranger 100 bp DNA Ladder
Product Info
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Product Info
Overview
Ready-to-use
Quantitative
Highly Stable
Precise
Eleven discrete fragments ranging from 50 bp to 1000 bp
Higher intensity reference band at 500 bp
The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.
Contents 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
PCR Ranger 100 bp DNA Ladder (Cat# 11300) – 100 loads
Ladder Properties: • Eleven discrete bands, ranging from 50 bp to 1,000 bp • Higher intensity band at 500 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
1000
91
2
900
66
3
800
61
4
700
51
5
600
43
6
500
70
7
400
28
8
300
23
9
200
30
10
100
22
11
50
15
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
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The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
