Usage: For isolating lactose-fermenting Gram-negative enteric bacilli.
Approximative Formula(Per Liter)
Pancreatic Digest of Gelatin 17g
Peptones(meat and casein) 3.0g
Lactose Monohydrate 10g
Sodium Chloride 5g
Bile Salts 1.5g
Agar 13.5g
Neutral Red 30mg
Crystal Violet 1mg
Final pH 7.1±0.2
Directions: Suspend 50g in 1 L of distilled or deionized water. Heat with frequent agitation to dissolve. Boil 1~2min. Distribute into flasks. Cool to 45~50°C and pour into sterile petri dishes.
Storage: Keep container tightly closed, store in a dry place, away from bright light.
Other Products
IVD3101 MagPure DNA Micro Kit
Product Info
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Product Info
Introduction
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
Applications
Second generation sequencing, PCR, real timePCR, etc.
Purification method
Monodisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
Body fluid : 10 – 200μl, Tissue : ≤10mg
Yield
10ng – 15μg
Elution volume
80 -100μl
Time per run
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
High purity – carboxyl magnetic beads with weak surface adsorption, getting higher purity
General – can be used for various clinical samples
Kit Contents
Contents
IVD3101
Purification Times
200
MagBind Particles
4.5 ml
Proteinase K
90 mg
Protease Dissolve Buffer
10 ml
Buffer ATL
60 ml
Buffer AL
60 ml
Buffer BD*
20 ml
Buffer BXW1*
110 ml
Elution Buffer
30 ml
Storage and Stability
Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Attogene’s Montmorillonite Bentonite Clay binds toxins with high affinity and high capacity. Montmorillonite has been shown to be the active ingredient in bentonites. The proposed mechanism of action of Attogene’s Montmorillonite Bentonite Clay is through the adsorption of toxins (mainly onto the interlayer spaces of montmorillonite). Attogene’s Montmorillonite Bentonite Clay is a common anti-caking agent in animal feeds to adsorb aflatoxins and diminish their bioavailability. Its composite for application in drug system can be formed through the utilization of anionic, cationic, and nonionic surfactants.
Bentonite is a promising rock of clay which is found in nature. It is a major source of montmorillonite in nature. It is a rock produced of highly colloidal and plastic clays mainly comprised of montmorillonite. In addition to montmorillonite, bentonite may compose of some amount of crystalline quarz, cristobalite, and feldspar. Montmorillonite nanoclay is used as a drug carrier system and as an additive. Its composite for application in drug system can be formed through the utilization of anionic, cationic, and nonionic surfactants. These are used to improve basal spacing resulting in organo clay to be utilised in drug loading and drug release.
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Promotes flocculation of cyanobacteria cell debris, binds residual phosphate compounds and promotes gradual settling of the cyanobacterial biomass on the sediment.
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s EBV TaqMan PCR Lyophilized Kit is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s EBV TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
