Usage: For isolating lactose-fermenting Gram-negative enteric bacilli.
Approximative Formula(Per Liter)
Pancreatic Digest of Gelatin 17g
Peptones(meat and casein) 3.0g
Lactose Monohydrate 10g
Sodium Chloride 5g
Bile Salts 1.5g
Agar 13.5g
Neutral Red 30mg
Crystal Violet 1mg
Final pH 7.1±0.2
Directions: Suspend 50g in 1 L of distilled or deionized water. Heat with frequent agitation to dissolve. Boil 1~2min. Distribute into flasks. Cool to 45~50°C and pour into sterile petri dishes.
Storage: Keep container tightly closed, store in a dry place, away from bright light.
Adenovirus Purification from any input – cell fraction or media fraction
Rapid purification within 2 to 4.5 hours
No specialized equipment needed (ultracentrifuge not required)
Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep
Purify adenovirus cell pellet from 1 mL of input per prep
Up to 25X sample concentration
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.
Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.
At least 3×1011 adenovirus particles as determined by qPCR At least 3×108 transducing units as determined by transduction assay
Input type
Cells, media
Input volume (Supernatant)
1 – 33.5 mL SN per prep (500 mL SN in total)
Input Volume (Cell pellet)
1 mL cell pellet per prep (15 mL in total)
Minimum elution volume
1.3 mL per prep
Time to complete purification
2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction
Yes
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Neisseria gonorrhoeae Quantified Bacterial DNA Standard
Product Info
Document
Product Info
Overview
Quantified standard to be used as a positive control or PCR quantification standard
Vigorously quantified using multiple methods
Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is the etiological agent of the sexually transmitted infection (STI) gonorrhoea, which globally causes an estimated 60 million new cases of gonococcal disease annually. It is second only to Chlamydia trachomatis as the most reported notifiable sexually transmitted disease. Infections with N. gonorrhoeae are primarily restricted to the mucus membranes of the endocervix, urethra, rectum, and pharynx. In females, gonorrhoea is a major cause of pelvic inflammatory disease and may lead to tubal infertility, ectopic pregnancy, and chronic pelvic pain, whereas in males it primarily causes urethritis. Importantly, these infections may often be asymptomatic, thereby contributing to further transmission and maintenance of the disease within populations.
Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard is prepared from pelleted bacteria grown on culture plates using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Neisseria gonorrhoeae.
Volume Provided – 250 µL DNA Quantity – 2 x 104 copies per µL
Storage Conditions Upon receipt, store Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
