Usages: For isolating , cultivating mold and yeast .
Principle: Potato flour leaching contribute various mold growth; glucose to provide energy; agar as medium coagulant; chloramphenicol inhibit the growth of bacteria.
Formulation(per liter): Infusion from potatoes 200g Glucose 20g Agar 15g Final pH 5.6 ± 0.2
How to use: 1.Suspend 40g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
Total Dietary Fiber Controls
Product Info
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Product Info
K-TDFC
SKU: 700004345
Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Content:
Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: Ambient, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Dietary Fiber
For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
Attogene’s Nano-clay Powder is comprised of thin layers and each layer has a thickness of one to a few nanometers length from a few hundred to several thousand nanometers. Nanparticles are utilized as fillers or additives in polymers for variety of desirable effects are receiving an increased interest for research and development. Different types of nanoparticles, such as nanocarbon, carbon nanotubes, nano-clays, and metal oxides, are recently employed to modify the polymer performance.
The viable interest is in the utilization of nano-clays for the alteration of polymeric material for numerous applications. This may be indicated from the increased commercial interest, and utilization of clay nanocomposites. It is an organic and hydrophilic. Moreover, the nanoclays are added in the polymers to increase the mechanical properties of polymers. In the nanocomposites, the permeability of the gases such as oxygen, carbon dioxide can be reduced by the addition of nanoclays. Nano clays have wide range of applications in food-packaging, pharmacy, used as a drug vehicle, textile industry etc.
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Promotes flocculation of cyanobacteria cell debris, binds residual phosphate compounds and promotes gradual settling of the cyanobacterial biomass on the sediment.
Cat.# 20106S, 20106L: Size range 450-750 bp (ideal for NGS library size selection)
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The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.
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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
DNA size selection with dual clean-ups.
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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
DNA size selection with single clean-up for >5 kb and >10 kb DNA.
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Features of DNA size selection and library size selection
High specificity and high recovery of size selection
11 selection ranges are available, including 5 ranges for NGS library size selection
50-100 bp
100-200 bp
200-500 bp
250-350 bp: ideal for illumina PE100 sequencing
300-450 bp: ideal for illumina PE150 sequencing
450-750 bp: ideal for illumina PE300 sequencing
500-1000 bp
1-3 kb
1-5 kb
>5 kb: ideal for long-read sequencing
>10 kb: ideal for long-read sequencing
Fast and simple
20-min protocol
No gel purification required
No columns required
No centrifugation required
Efficient removal of contaminants and unwanted components
