For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection.
Formula (per liter)
Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2
How to use
1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples.
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
D3163 HiPure HP Plant DNA Maxi Kit
Product Info
Document
Product Info
Introduction
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 3g plant and fungal tissue
Applications
PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Various plant samples (including conventional, polysaccharides and polyphenols)
This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
Broad spectrum – suitable for extracting DNA from various plant samples
Fast – several samples can be extracted in 30 minutes by silica technology
High purity – high quality DNA, completely remove inhibitors
High yield – silica technology can achieve the highest yield
Kit Contents
Contents
D316302
D316303
Purification Times
10 Preps
50 Preps
RNase A
20 mg
90 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer PAL
180 ml
900 ml
Buffer GWP
150 ml
800 ml
Buffer GW2
25 ml
200 ml
Buffer AE
30 ml
120 ml
HiPure DNA Maxi Columns II
10
50
50 ml Collection Tubes
20
100
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.
CP0202J Triple Wrapped Irradiated Plate-Tryptic Soy Agar with B-lactamase
Product Info
Document
Product Info
Introduction
Usages: For monitoring and detection of surface of equipment and personnel hygiene which have disinfectant or antibiotics.
Advantage: 1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life. 2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability. 3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation. 4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit.. 5.This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.