Peptone, yeast extract powder, beef extract powder provides carbon and nitrogen sources, vitamins and minerals; lactose as fermentable sugars; agar as medium coagulant; potassium hydrogen phosphate as buffer.
Formulation(per liter):
Peptone 10g
Yeast extract powder 5g
Beef extract powder 5g
Lactose 10g
Agar 15g
Dipotassium hydrogen phosphate 3.5g
Sodium sulphite 5g
Fuchsin 1g
Final pH 7.2 ± 0.2
How to use:
1.Suspend 54.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, autoclave at 115 for 20min. Cool to 50 and pour into petri dishes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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14 Month Validity Isothermal Amplification Kit NFO Keep Away From Light Keep Away From Light
Product Info
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Product Info
Product Description
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Product image shows functional testing of Protein A 40nm colloidal gold on a lateral flow test. Protein A 40nm colloidal gold was incubated in lateral flow running buffer alone or in the presence of a limiting amount of monoclonal antibody that targets the chemical-BSA conjugate. The gold was then applied to the sample port of a cassette containing a lateral flow strip sprayed with a chemical conjugate (Chemical-BSA) on the test line and a goat anti mouse antibody as the control line. The data demonstrates the specificity and robustness of the Protein A 40nm colloidal gold.
Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
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Functionally tested Protein A conjugated gold
Perfect for Lateral Flow Development and use in final product
40nm gold particles conjugated to Protein A are offered in 10 OD 1mL size. If a different OD or amount is required please
This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
Details
Specifications
Features
Specifications
Main Functions
Isolation gDNA from biological sample and remove host DNA
Applications
PCR, southern blot and enzyme digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Culture medium, swab, parasitic blood, tissue, sputum, etc.
This product is based on silica Column purification. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High concentration – the host DNA is digested by dnase, without contamination of host DNA
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Wide applicability – it can be used in blood, tissue, intestinal contents and other samples
Kit Contents
Contents
D314802
D314803
Purification Times
50 Preps
250 Preps
Hipure DNA Mini Columns I
50
2 x 125
2ml Collection Tubes
50
2 x 125
2ml beads Tubes
50
250
Buffer DRB
15 ml
60 ml
Buffer ES
6 ml
30 ml
Reagent DX
0.5 ml
1 ml
Buffer DL
30 ml
120 ml
Buffer GW1
22 ml
110 ml
Buffer GW2
12 ml
50 ml
DNase I (Powder)
6 mg
30 mg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
5 ml
15 ml
Buffer AE
15 ml
60 ml
Storage and Stability
DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
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This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
