Genomic DNA Extraction Kit (HMW, Magnetic Beads)

The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.

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Cat.# 50014 Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads)
Cat.# 50015 Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads)
Cat.# 50016 Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads)

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The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.

Features

• High molecular weight DNA: 50 kb to 150 kb
• High purity
• Simple magnetic beads method
  • No centrifuge needed
  • No column needed
  • No vacuum needed

A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.

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Cultured Cell samples

Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

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Blood samples

Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

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Tissue samples

Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Overview

• Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
• No hazardous chemicals required (e.g. phenol or chloroform)
• Effective lysis with Proteinase K and optional lysozyme treatment
• Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
• Wide compatibility with a variety of food products for GMO-DNA isolation
• Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)

This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.

This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping.  

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice)	200 mg 1 mL to 1.5 mL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Samples Tested by PCR

Food Materials	Samples that Have Been Tested by PCR
Plant related	Cereal, Jam, Chocolate, Spices, Sauce
Animal related	Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage)
Dairy product	Milk, Yogurt, Cheese
Pathogens (enriched from food samples)	E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk

Component	Cat. 54500 (50 preps)
Lysis Buffer L	60 mL
Binding Buffer I	7 mL
Buffer SK	30 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Proteinase K	1 vial
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1