For the cultivation of E. coli in fermentation and molecular genetic studies.
Principle and Interpretation
Peptone provides nitrogen . Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Glucose provides carbon.Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent.
Formulation
Ingredients
/liter
Peptone
10.0g
Yeast extract
5.0g
Sodium chloride
5.0g
Glucose
1.0g
Agar
15.0g
pH7.0±0.2 at 25°C
Preparation
Suspend 36g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains
Growth
Escherichia coli ATCC25922
good
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Other Products
Bis-propargyl-PEG10
Product Info
Document
Product Info
Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
No sample splitting or need to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
