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Heat Labile Exonuclease I

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Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.

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Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C. 

HL-ExoI is used for degradation of ssDNA such as primers and oligos. It is also ideal for treatment of sensitive samples and useful in the development of novel molecular diagnostics applications.

Key Features

  • 3’-5’ exonuclease specific for single stranded DNA
  • High activity at 25 – 37°C
  • Easily heat-inactivated by 1 min incubation at 80°C, or 15 min at 60°C
  • Moderate salt tolerance

Application

Removal of primers post-PCR prior to DNA sequencing or SNP detection

Figures

Properties

Quality Control

ArcticZymes is dedicated to the quality of our products. We manufacture all products at our ISO 13485 certified facility in Norway.

Other Products

ITS2 Library Preparation Kit for Illumina

Overview

  • Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
  • Simple and quick workflow: library could be prepared in less than 4 hours
  • Component of Norgen’s metagenomics workflow
  • A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The ITS2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS2 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The ITS2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS2 region of the fungal DNA. The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.16S Library Prep

Details

Supporting Data

Figure 1 / 3

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Figure 1. Amplicons generated by ITS2 amplicon primers, cleaned and run on a 2% agarose gel.
Figure 1. Amplicons generated by ITS2 amplicon primers, cleaned and run on a 2% agarose gel.
Figure 2. Libraries generated by indexing PCR, cleaned and run on a 2% agarose gel.
Figure 3. Bioanalyzer traces of ITS2 libraries generated by indexing PCR, cleaned and run on a high sensitivity DNA chip.

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Minimum amount of starting material:2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:4 hours

Storage Conditions and Product Stability
Norgen’s ITS2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components will remain stable for at least 1 year when stored at the specified storage conditions.

GeneAb™ E-cadherin

Description

Specifications

CloneIHC564
SourceMouse Monoclonal
Positive ControlBreast
Dilution Range1:200

m-PEG4-DBCO

m-PEG4-DBCO is a click chemistry reagents which can enable so called copper-free Click Chemistry with azide to form a stable triazole. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.