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Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
HL-ExoI is used for degradation of ssDNA such as primers and oligos. It is also ideal for treatment of sensitive samples and useful in the development of novel molecular diagnostics applications.
Key Features
- 3’-5’ exonuclease specific for single stranded DNA
- High activity at 25 – 37°C
- Easily heat-inactivated by 1 min incubation at 80°C, or 15 min at 60°C
- Moderate salt tolerance
Application
Removal of primers post-PCR prior to DNA sequencing or SNP detection
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