Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
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Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
HL-ExoI is used for degradation of ssDNA such as primers and oligos. It is also ideal for treatment of sensitive samples and useful in the development of novel molecular diagnostics applications.
Key Features
3’-5’ exonuclease specific for single stranded DNA
High activity at 25 – 37°C
Easily heat-inactivated by 1 min incubation at 80°C, or 15 min at 60°C
Moderate salt tolerance
Application
Removal of primers post-PCR prior to DNA sequencing or SNP detection
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No phenol extraction step
Includes DNase for optional on-column DNA removal
Isolated RNA is of the highest quality and integrity
Isolate a diversity of RNA species
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
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Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
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5 slices of < 20 µm thick paraffin 25 mg of unsectioned block
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