Nineteen discrete fragments ranging from 100 bp to 10000 bp
Higher intensity reference bands at 500 bp, 1000 bp and 5000 bp
The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.
Contents: 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
HighRanger Plus 100 bp DNA Ladder (Cat# 12000) – 100 loads
Ladder Properties:
Nineteen discrete fragments ranging from 100 bp to 10000 bp
Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
10000
42
2
8000
33
3
6000
25
4
5000
42
5
4000
35
6
3000
27
7
2500
22
8
2000
18
9
1500
13
10
1000
40
11
900
28
12
800
25
13
700
34
14
600
19
15
500
44
16
400
13
17
300
18
18
200
13
19
100
10
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Other Products
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits
Product Info
Document
Product Info
Overview
Columns bind proteins of interest while endotoxins flow through
Reduce endotoxin levels to less than 0.01 EU/µg of protein
Proteins are desalted
Greater than 95% protein recovery
Concentrate protein samples and remove detergents at the same time
Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Component
Cat. 22800 (25 preps)
Cat. 22200 (4 preps)
Binding Buffer J
8 mL
8 mL
Binding Buffer N
4 mL
20 mL
Wash Solution M
50 mL
130 mL
Wash Solution CIP
20 mL
60 mL
Wash Solution N
30 mL
130 mL
Wash Solution NIP
20 mL
60 mL
Elution Buffer G
6 mL
20 mL
Endotoxin Removal Solution
1.5 mL
1.5 mL
Protein Neutralizer EF
2 mL
2 mL
Maxi Spin Columns (assembled with Collection Tubes)
–
4
Mini Spin Columns
25
–
Collection Tubes
25
–
Maxi Spin Columns (assembled with Collection Tubes)
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
30nm Colloidal Gold for Lateral Flow is a highly stable and uniform 30 nm gold nanoparticles can be supplied in a range from 1 OD to 100 OD. The quality and performance of a conjugate is critical to successful lateral flow test manufacturing. Our products are made in Austin TX and produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
Bulk pricing and manufacturing supply contracts available.
Functionally Tested in Lateral Flow
Specifications 1OD 30nm Gold (1L)
Number of particles/mL
1.3-2 x 1011
Gold Concentration (mg/mL)
4.2-4.7 x 10-2
Molar Concentration (moles/liter)
2.2-3.3 x 10-10
Particle Diameter
30 nm /-1.5
Document
Number of particles/mL 1.3-2 x 1011 • Gold Concentration (mg/mL) 4.2-4.7 x 10-2 • Molar Concentration (moles/liter) 2.2-3.3 x 10-10 • Particle Diameter 30 nm /-1.5
