Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum
Detail
20 tests
Name of Product
HIT – Heparin-Induced Thrombocytopenia Test (20)
Catalog Number
MQHIT 1
Short Info
Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum
This product is only available in Germany!
Method/Platform
lateral flow, immunoassay
Range/Assay Sensivity
Test Principle
IgG antibodies are resposible for the heparin induced thrombocytopenia.
Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.
The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.
Anti-Hairy Cell Leukemia stains various B-cells in the follicular mantle zone and virtually all cases of hairy cell leukemia. It also stains some high grade B-cell lymphomas.
40nm Colloidal Gold for Lateral Flow is a highly stable and uniform 40 nm gold nanoparticles can be supplied in a range from 1 OD to 100 OD. The quality and performance of a conjugate is critical to successful lateral flow test manufacturing. Our products are made in USA and produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
Bulk pricing and manufacturing supply contracts available.
Functionally Tested in Lateral Flow
Specifications 1OD 40nm Gold (1L)
Number of particles/mL
5.2-7.2 x 1010
Gold Concentration (mg/mL)
3.7-4.2 x 10-2
Molar Concentration (moles/liter)
8-12 x 10-11
Particle Diameter
40 nm /- 1.5
Document
Number of particles/mL 5.2-7.2 x 1010
• Gold Concentration (mg/mL) 3.7-4.2 x 10-2
• Molar Concentration (moles/liter) 8-12 x 10-11
• Particle Diameter 40 nm /- 1.5
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Plasmid Mini Preparation, gDNA/ RNA Extraction,DNA/RNA Clean Up
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/B, 3 layers
Membrane aperture
1.0μm
Maximum binding yield of plasmid
30 μg
Maximum yield of alcohol mediated Binding
200 μg
Single liquid carrying capacity of column
800 μl
Minimum elution volume
30 μl
Withstand centrifugal force
16,000 x g
Centrifuge
Small high speed centrifuge (2ml)
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13111
HiPure RNA Mini Column (3 x GF/B)with 2ml Collection Tubes
1000/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
