Name of Product
Hop Resistance Gene-Screen
Catalog Number
MGScHOR 1
Short Info
This test discovers hop resistance genes in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Detail
Name of Product
Hop Resistance Gene-Screen
Catalog Number
MGScHOR 1
Short Info
This test discovers hop resistance genes in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Brief Instructions
The PCR reagents and the samples are prepared.
After the addition of the sample to the PCR reagents, the PCR is started.
The resulting PCR products are detected by a simple lateral flow test Strip
Formaldehyde is an organic compound with the formula CH2O. It is mainly used in the production of industrial resins but has been found to be used as a preservative, disinfectant and biocide. Because of its toxicity and volatility formaldehyde poses a significant risk to human health. Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
Measuring range / color- Number of scale graduation
Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.6ml
Elution volume
Time per run
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
1292750
12927200
Purification Times
50 preps
200 preps
MagPure Particles F
1.2 ml
4.5 ml
MagBind Particles
1.2 ml
4.5 ml
Proteinase K
24 mg
90 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer MLB
30 ml
120 ml
Buffer GDP
15 ml
60 ml
Buffer MAW1
40 ml
250 ml
Buffer MW2*
20 ml
50 ml
Elution Buffer
15 ml
60 ml
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
tert-Butyl (2-(prop-2-yn-1-yloxy)ethyl)carbamate is crosslinker consisting of a propargyl group and a t-Boc protected amine group. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The t-Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research use only.
Document
tert-Butyl (2-(prop-2-yn-1-yloxy)ethyl)carbamate is crosslinker consisting of a propargyl group and a t-Boc protected amine group. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The t-Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research use only.
