Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced band sharpness
Better resolution of small proteins
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Document
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.
Details
Specifications
Features
Specifications
Main Functions
Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt SPRISelect)
Applications
Prepare DNA library for second generation sequencing
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
DNA products, restriction endonuclease systems, or other enzymatic reaction solution
Sample amount
Appropriate
Recovery
80%
Operation time
≤50 minutes
Principle
The MagSelect method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger to paramagnetic beads. Excessprimers, nucleotides, salts, and enzymes can be removed using a simple washing procedure. The result is a more purified PCR product.
Advantages
High recovery – up to 80%
High throughput – using magnetic beads purification technology
Kit Contents
Contents
XP-5
XP-50
XP-500
MagSelect Beads
5 ml
50 ml
500 ml
Storage and Stability
MagSelect XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
Document
This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.