Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
SNPsig® EscapePLEX™ SARS-CoV-2 also incorporates the two gene (ORF1ab and M Gene) assay to provide a confirmatory detection of SARS-CoV-2.
DETECTION PROFILE: The E484K, K417N, K417T and P681R mutations which are found in Beta, Gamma, Delta and Delta Plus Variants of Concern
For general laboratory and research use only.
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Rapid detection of specific detection profiles High priming efficiency Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions
Bis-propargyl-PEG18 has two propargyl groups at both ends. These propargyl groups reacts with azide compounds or biomolecules to form stable triazole linkages under the catalyzation of copper. The hydrophilic PEG units enhance the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Bis-propargyl-PEG18 has two propargyl groups at both ends. These propargyl groups reacts with azide compounds or biomolecules to form stable triazole linkages under the catalyzation of copper. The hydrophilic PEG units enhance the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
Details
Specifications
Features
Specifications
Main Functions
Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen RNAClean XP)
Applications
Advanced applications, such as sequencing, genechip, fluorescence quantification, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
RNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount
20-100µl
Recovery
90%
Elution volume
≥20μl
Operation time
≤50 minutes
Principle
RNA Clean utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads. Excess oligonucleotides, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
High recovery – recovery of RNA up to 90%
High throughput – using magnetic beads purification technology
Store at 4℃ upon arrival, for up to 12 months. For best results shake the reagent well until all of the beads are completely in suspension and aliquot RNAClean Beads into RNase free containers. Do not pour remaining reagent back into the storage container. Mix RNAClean Beads well before use. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
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The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
