Human Metapneumovirus

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Detail

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Other Products

DBCO-PEG4-triethoxysilane

Product Info

Document

Product Info

Document

Cerillo Alto Plate Reader Adapter

Product Info

Document

Product Info

Document

Saliva DNA Isolation 96-Well Kit

Product Info

Document

Product Info

Overview

Sample collection is non-invasive and painless
Fast and easy high throughput processing using 96-well plates
Isolate high quality genomic DNA
Effective removal of PCR inhibitors
Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva. The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). It is fully compatible to both vacuum and centrifugation protocols.

Background

Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.

Details

Supporting Data

Figure 1 / 2

Figure 1. Consistent DNA Isolation

Figure 1. Consistent DNA Isolation

Figure 2. Real-Time PCR Consistency

Click for expanded view

Kit Specifications
Binding Capactity Per Well	50 μg
Maximum Loading Volume Per Well	600 μL
Size of DNA Purified	All sizes
Time to Complete 96 Purifications	40 minutes

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. RU35200 (192 preps)
Lysis Buffer F	100 mL
Proteinase K in Storage Buffer	4 mL
Binding Buffer B	40 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Plate	2
96-Well Collection Plate	2
Adhesive Tape	4
96-Well Elution Plate	2
Product Insert	1

Document