Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Small RNA Library Prep Kit for Illumina
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Product Info
Overview
Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA
Simple and quick workflow: library could be prepared in less than 5 hours
No gel purification for selected types of samples
Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)
Complements Norgen’s Best-in-Class Total RNA (including microRNA) Purification Technology
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.
Storage Conditions and Product Stability Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.
Step
Component
Cat. 64600 (24 preps)
3′ AdaptorLigation to Template RNA
3′ Adaptor
30 µL
3′ Adaptor Ligation Master Mix
320 µL
T4 RNA Ligase 2 (Truncated)
35 µL
5′ Adaptor Ligation
5′ Adaptor
30 µL
5′ Adaptor Ligation Master Mix
320 µL
T4 RNA Ligase 1
35 µL
cDNA Synthesis from Ligated RNA Product
Reverse Primer
30 µL
cDNA Synthesis Master Mix
220 µL
TruScript ReverseTranscriptase
35 µL
PCR Amplification
2x NGS PCR Master Mix
1.32 µL
PCR Reverse Primer
81 µL
Forward Index Primer
Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610)
Floor Type High Speed & Low Speed Universal Refrigerated Centrifuge
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Product Info
GL20 Technical Parameter:
Max. Speed
21000rpm
Max. RCF
30910×g
Max. Capacity
4×800ml
Time Range
1~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 60
Temperature
-20℃~40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
680×540×830mm
Net Weight(Kg)
170KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for GL20
Order No
Rotor
Rotor Max Speed(r/min)
Volume(ml)
RCF(×g)
G20-1
Swing Rotor
4000
4×800ml
3450
G20-2
Microplate rotor
4000
4×4×96 well
2940
G20-3
Microplate rotor
4000
2×4×96 well
3210
G20-4
Microplate rotor
4000
2×3×48 well
2300
G20-5
Swing rotor
4000
4×40×7ml vacuum tube
3270
G20-6
4000
4×40×5ml vacuum tube
2890
G20-7
4000
4×28×10ml vacuum tube
3130
G20-8
Swing Rotor
4000
Fat bottle
3830
G20-9
Fixed Rotor
16000
4×8PCR
15760
G20-10
Fixed Rotor
15000
6×8PCR
21420
G20-11
Fixed Rotor
16000
8×8PCR
17480
G20-12
Fixed Rotor
15000
12×8PCR
22930
G20-13
Fixed Rotor
15000
40×0.5ml
22920
G20-14
Fixed Rotor
21000
12×1.5ml
30910
G20-15
Fixed Rotor
16000
24×1.5ml
23440
G20-16
Fixed Rotor
14000
30×1.5ml
20800
G20-17
Fixed Rotor
13000
48×1.5ml
17930
G20-18
Fixed Rotor
16000
16×5ml
22020
G20-19
Fixed Rotor
16000
6×10ml
21500
G20-20
Fixed Rotor
15000
12×10ml
22680
G20-21
Fixed Rotor
13000
16×10ml
19490
G20-22
Fixed Rotor
13000
8×15ml
17790
G20-23
Fixed Rotor
11000
12×15ml
14330
G20-24
Fixed Rotor
5000
24×15ml
3500
G20-25
Fixed Rotor
5000
30×15ml
3830
G20-26
Fixed Rotor
14000
6×30ml
19060
G20-27
Fixed Rotor
13000
6×50ml(conical)
18840
G20-28
Fixed Rotor
13000
6×50ml(round)
18730
G20-29
Fixed Rotor
5000
12×50ml
3860
G20-30
Fixed Rotor
4000
24×50ml
2970
G20-31
Fixed Rotor
13000
4×85ml
18940
G20-32
Fixed Rotor
12000
4×100ml
14850
G20-33
Fixed Rotor
10000
6×100ml
11380
G20-34
Fixed Rotor
4000
12×100ml
2970
G20-35
Fixed Rotor
12000
24 capillaries
15800
G20-36
Swing Rotor
15000
4×5ml
19920
G20-37
Vertical rotor
16000
16×5ml
16450
G20-38
Vertical rotor
14000
8×30ml
19750
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Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.