Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
Aluminum Block – 96-well PCR Plate
Product Info
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Product Info
The Opentrons 96-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold full, half, and non skirted PCR plates as well as strip tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
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The Opentrons 96-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold full, half, and non skirted PCR plates as well as strip tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 1 year under recommended storage conditions
Analyte:
L-Rhamnose
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
5 to 100 µg of L-rhamnose per assay
Limit of Detection:
~ 1.2 mg/L
Reaction Time (min):
~ 5 min at 25oC or ~ 4 min at 37oC
Application examples:
Hydrolysates of plant material and polysaccharides, culture media / supernatants and other materials.
Method recognition:
Novel method
The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.
L-Rhamnose occurs naturally in the L-form and is commonly present as a component of the carbohydrate moiety of eukaryotic glycoproteins and in plant cell wall polysaccharides. The most abundant occurrence of L-rhamnose is within the pectic fraction of plant cell wall polysaccharides. L-Rhamnose is commonly used as a non-metabolisable marker along with lactulose for dual-permeability testing in the diagnosis of intestinal diseases such as Crohn’s disease or coeliac disease.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 2 years after preparation
Only test kit available
Simple format
Rapid reaction (~ 5 min at 25oC or ~ 4 min at 37oC)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture. Recommend for High copy vector, Low elution volume, High concentration
Applications
Cell transfection, animal injection, etc.
Purification method
Midi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
High copy plasmid vector
Sample amount
25-50ml LB
Yield
10-500μg
Elution volume
≥0.1ml
Time per run
≤60 minutes
Liquid carrying volume per column
4ml
Binding yield of column
250μg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High concentration – easily obtain concentrations >1000ng/μl
Small amount large extraction – using a small amount of column to achieve a large extraction yield (500μg)
Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection
Kit Contents
Contents
P123102
P123102B
Purification Times
50 Preps
50 Preps
RNase A
30 mg
30 mg
Buffer E1
150 ml
150 ml
Buffer E2
150 ml
150 ml
Buffer E3
150 ml
150 ml
Buffer E4
150 ml
150 ml
Buffer E5
150 ml
150 ml
Buffer PW2*
50 ml
50 ml
Elution Buffer
30 ml
30 ml
MaxPure EF Mini Column
50
50
2 ml Collection Tubes
50
50
Lysate Clear Midi Syringe
50
50
Extend Tubes
50
50
50ml Centrifuge Tubes
50
Support Tubes
50
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
