The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.
Detail
Name of Product
HybriDetect Cassette
Catalog Number
MGHC 1
Short Info
The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.
Please note, that you may have to pay country-specific taxes and duties.
Method/Platform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
10 pmol/LFA – 1 fmol/LFA
Test Principle
Milenia HybriDetect Cassette is a ready-to-use, universal test unit, which is based on the lateral flow technology using gold nanoparticles. The test unit is designed to develop qualitative or semi-quantitative rapid test systems for several analytes such as proteins, antibodies, or gene amplicons. The user needs to develop an analyte-specific solution or reaction (PCR, RPA, LAMP, CRISPR/Cas, …), which contains a first detector (e.g. antibody, antigen, specific probe) labelled with FITC or FAM and a second one (e.g. antibody, primer) labelled with biotin.
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
≤6 slices
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Fast – several samples can be extracted in 120 minutes by column method
Safe – no phenol chloroform extraction required
Kit Contents
Contents
IVD3022
Purification Times
200 Preps
MagBind Particles
4.5 ml
Proteinase K
100 mg
Protease Dissolve Buffer
6 ml
DNase I
4 x 600 µl
DNase Buffer
30 ml
Buffer DPS
200 ml
Buffer FRL
40 ml
Buffer AL
40 ml
Buffer MW1*
110 ml
Buffer MW2*
2 x 50 ml
Nuclease Free Water
30 ml
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Document
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
PC Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. PEG spacer provides better solubility to the labeled molecules in aqueous media. Captured biomolecules can be efficiently photoreleased using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
PC Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. PEG spacer provides better solubility to the labeled molecules in aqueous media. Captured biomolecules can be efficiently photoreleased using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
