Hydroxy-Amino-bis(PEG2-propargyl)

Bioprocessing with Salt Active Nucleases  – High Salt Conditions

OverView

For SAN HQ, SAN HQ ELISA Kit, and now SAN HQ GMP

SAN HQ GMP is biochemically identical to SAN HQ but produced under GMP conditions.

Applications

• Purification of biologics from residual nucleic acids in biopharma manufacturing
• Purification of recombinant proteins and enzymes for research and diagnostic use
• Removal of unwanted nucleic acids contamination in molecular biology reagents in challenging conditions
• Reduction of viscosity in biological samples during production and automation
• Vaccine manufacturing and viral vector preparation
• DNA removal in high-salt lysates

SAN HQ  – Peak performance at high salt conditions

Salt Active Nuclease High Quality (SAN HQ) is a Bioprocessing Grade nuclease developed as the most efficient solution for removal of both single and double stranded DNA and RNA at high salt conditions. 

This nonspecific endonuclease has peak activity at salt concentrations between 400 – 700 mM (Fig. 1)

Non-enveloped viruses like Adenoviruses and Adeno-Associated Viruses (AAV’s) are inherently more robust with two distinct advantages: 1) They exhibit higher tolerance to additives like salt and detergents and 2) their production often involves the lysis of host cells, allowing for harvesting non-secreted vectors. 

For Adeno-Associated Viruses (AAVs), which are often harvested from crude cell lysate, the high salt tolerance of SAN HQ is particularly beneficial. Salt is typically added to such lysates to reduce viral aggregation, facilitating more effective nuclease action to digest residual DNA.

SAN HQ’s is engineered for optimum activity in these high salt environments ensuring that you achieve unparalleled DNA removal without compromising the integrity of these robust viral vectors.

Key Benefits

• Optimized Residual DNA Removal: Ensures efficient degradation of residual DNA in high-salt conditions, meeting stringent quality requirements for biologics and vaccines.
• Boosted AAV Vector Purification: Enhances the purification process for adeno-associated viral vectors in high-salt conditions, improving quality and yield.
• Streamlined Workflow: Eliminates the need for desalting stages, simplifying the bioprocessing protocol and saving time and resources.
• Enable High-Throughput Processes: Facilitates scale-up and automation by working effectively in high-salt environments, increasing operational throughput.
• Potential Surge in Virus Yield: Operates under conditions that may boost the titer yield of AAV production, potentially enhancing overall viral yield.
• Economized Enzyme Usage: Reduces the need for excess enzyme and additional process adjustments, resulting in significant cost savings.
• Minimized Risk of Process Disruptions: Offers reliable performance in various high-salt bioprocessing conditions, reducing the likelihood of disruptions due to enzyme inhibition.
• Reliability: Provides consistent enzyme activity in challenging high-salt conditions, adding a layer of predictability and dependability to your operations.
• Broader Applicability: Versatile enough to be used in a wide range of viral vector systems, expanding your research and production capabilities.
• Enhanced Viral Stability: High-salt levels stabilize viral vectors, and SAN HQ operates effectively in these conditions, maintaining high yield and quality.
• Host Cell Lysis: Facilitates efficient lysis of host cells in high-salt conditions, optimizing the harvest of both secreted and non-secreted viral vectors.

Key Features 

• High purity (≥ 98%)
• No protease detected
• Supplied with extended product documentation
• Compatible with SAN HQ ELISA

The Challenge in Removing Host Cell Chromatin Impurities 

In bioprocessing, the primary role of a nuclease is to efficiently digest and fragment host-cell DNA into sufficiently small pieces, facilitating its removal during downstream processing. While most nucleases can effectively degrade naked DNA into tiny fragments under optimal conditions—as demonstrated by M-SAN HQ and SAN HQ, which can digest dsDNA into fragments smaller than 6 nt—the reality in bioprocessing is more complex. (See fig. 5)

The DNA targeted for removal often exists as chromatin, embedded in a complex matrix containing remnants of the lysed host cell as well as large amounts of the therapeutic product.The product may or may not have an affinity for the chromatin you aim to remove.

High salt is often applied to mitigate issues like aggregation. The real challenge lies in a nuclease’s ability to efficiently fragment chromatin under these more complicated, high-salt, conditions—not merely degrading naked DNA under ideal circumstances.

See Case Study on Efficient Fragmentation: Superior Chromatin Digestion Before Flow-Through Purification

SAN HQ ELISA Kit

SAN HQ ELISA kit is developed for the detection and quantification of SAN HQ and SAN  HQ GMP. The kit is designed as a classical sandwich ELISA, with two monoclonal antibodies specific towards SAN HQ nuclease (fig 6).

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Features

• Sensitive: 0.4 – 25.6 ng/ml
• Precise: RSD ≤ 15%
• Accurate: 100% ± 15%
• Stability: 12 months when stored between +2°C to +8°C

For SAN HQ, SAN HQ ELISA Kit, and now SAN HQ GMP
SAN HQ GMP is biochemically identical to SAN HQ but produced under GMP conditions.

Compact and stylish designed, the BioShake XP high-speed microplate mixer lets you perform all your standard mixing runs with a minimum of adjustments. The XP offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.

Small. Light. Efficient. Quiet.

• Ideal for microplates & PCR plates
• High-precision mixing from 200 up to 3,000 rpm
• Universal spring clamps for easy fixation
• Wide range of adapters
• Stylish, fire resistant aluminium housing

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ADVANCED TECHNOLOGY

Compact & stylish designed

Mixing tasks in microplates can be realized fast & safe with using adjustable speed of 200 up to 3,000 rpm.

The BioShake mixing-technology is obviously more robust, vibration free and needs less maintenance compared to classical mixers.

The first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.

GENTLE MIXING OF SAMPLES

By planar orbital motion

German designed and manufactured, BioShake XP plate mixer offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.

The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.

Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.

COMFORT

Simple intuitive operation and programming

The BioShake XP is operated from the front face of the enclosure which includes touch buttons and a display. 

Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.

The short mix button allows short and fast mixing in between.

The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time and mixing frequency.

UNIVERSAL SPRING CLAMPS

Suitable for all microplates

In the corners eight spring clamps are inserted for easy fixation of skirted microplates and deep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.

For flexible and deformable PCR plates we recommend using of the related PCR adapter.

Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.

UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY

First-class finished aluminium housing

Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment.  Therefore, the first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.

Safety of the user and ease of use are clearly a priority for us.

EXCHANGABLE ADAPTERS

For a wide range of applications

The BioShake XP comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple. 

Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels.

MADE IN GERMANY

High-end instruments for daily routine

A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.

The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.

Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing

K-ACHDF

SKU: 700004259

100 assays of each component

Content:	100 assays of each component
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Available Carbohydrates, Dietary Fiber
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Limit of Detection:	0.5 g/100 g
Reaction Time (min):	~ 78 min
Application examples:	Food ingredients, food products and other materials.

The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.

See more of our dietary fiber products.

Advantages

• Very cost effective 
• All reagents stable for > 2 years after preparation 
• High purity / standardised enzymes employed 
• Only kit available 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Simple format

The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.